Molecular characterization and function of beta-N-acetylglucosaminidase from ridgetail white prawn Exopalaemon carinicauda

doi:10.1016/j.gene.2018.01.046

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Molecular characterization and function of beta-N-acetylglucosaminidase from ridgetail white prawn Exopalaemon carinicauda
	Sun, Yuying 1,3; Zhang, Jiquan 1,2; Xiang, Jianhai2,4
刊名	GENE
	2018-03-30
卷号	648 页码:12-20
关键词	beta-N-acetylglucosaminidase Exopalaemon carinicauda Pichia pastoris Recombinant expression
ISSN号	0378-1119
DOI	10.1016/j.gene.2018.01.046
通讯作者	Zhang, Jiquan(zhangjiquan@qdio.ac.cn)
英文摘要	Chitin degradation is catalyzed by a two-component chitinolytic enzyme system, chitinase and beta-N-acetylglucosaminidase (NAGase). In this paper, the full-length cDNA sequence encoding NAGase (EcNAG) was obtained from Exopalaemon carinicauda. The deduced amino acid sequence of EcNAG open reading frame (ORF) contained one Glycohydro_20b2 domain and one Glyco_hydro_20 domain. Based on the cDNA sequence, the genomic structure of EcNAG was characterized and it was composed of six exons and five introns. EcNAG mRNA majorly expressed in the hepatopancreas and epidermis. During the molting stages, EcNAG mRNA expression was well-regulated and its expression reached the highest level at the molting stage E. In addition, EcNAG was recombinant expressed in Pichia pastoris and the partial enzymatic characterization of recombinant EcNAG was confirmed. After being challenged with Vibrio parahaemolyticus and Aeromonas hydrophila, the expression of EcNAG was up-regulated significantly at 6 h and reached the peak at 12 h. And then, the expression began to down-regulated and came to the normal level at 72 h. It is helpful to research the relationship between the molt related hormones and chitinlytic enzymes.
资助项目	National Natural Science Foundation of China[31172449] ; National Natural Science Foundation of China[41376165] ; National High Technology Research and Development Program of China[2012AA10A401]
WOS研究方向	Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000426410200003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158059]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Zhang, Jiquan
作者单位	1.Hebei Univ, Coll Life Sci, Baoding 071002, Hebei, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Huaihai Inst Technol, Coll Marine Life & Fisheries, 59 Cangum Rd, Lianyungang 222005, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yuying,Zhang, Jiquan,Xiang, Jianhai. Molecular characterization and function of beta-N-acetylglucosaminidase from ridgetail white prawn Exopalaemon carinicauda[J]. GENE,2018,648:12-20.
APA	Sun, Yuying,Zhang, Jiquan,&Xiang, Jianhai.(2018).Molecular characterization and function of beta-N-acetylglucosaminidase from ridgetail white prawn Exopalaemon carinicauda.GENE,648,12-20.
MLA	Sun, Yuying,et al."Molecular characterization and function of beta-N-acetylglucosaminidase from ridgetail white prawn Exopalaemon carinicauda".GENE 648(2018):12-20.