Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus

doi:10.1038/s41598-018-20065-1

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus
	Yue, Xin 1; Huan, Pin 1; Hu, Yonghua 1; Liu, Baozhong1,2
刊名	SCIENTIFIC REPORTS
	2018-01-30
卷号	8 页码:13
ISSN号	2045-2322
DOI	10.1038/s41598-018-20065-1
通讯作者	Liu, Baozhong(bzliu@qdio.ac.cn)
英文摘要	A worldwide increase in the reports of diseases affecting marine organisms has paralleled the climate warming over the past few decades. In this study, we applied omics to explore the mechanisms underlying thermo-linked epizootics, by comparing both the transcriptome- and proteome-wide response of turbots to a mimic pathogen (poly I:C) between high temperature and low temperature using a time-course approach. Our results showed that myeloperoxidase (MPO) and insulin were differentially expressed transcripts shared by all five time-points post poly I:C-injection between high and low temperature and also had a consistent expression trend as differentially expressed proteins at 24 h post injection. Combined with other data, it was suggested that the elevated temperature enhanced neutrophil-mediated immunity and the resultant MPO-mediated oxidative stress, which lasted for at least 5 days. The contents of malondialdehyde and protein carbonyls, markers of oxidative damage for lipids and proteins, respectively, were compared between different temperature groups, and the results further implied the emergence of oxidative damage under high temperature. It was also suggested that metabolism disorder likely occur considering the sustained expression changes of insulin. Hence, prolonged MPO-mediated oxidative stress and metabolic disorder might be involved in the thermo-linked epizootic.
资助项目	National Basic Research Program of China[2012CB114400] ; Scientific and Technological Innovation Project from Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02]
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000423508900067
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/157998]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Baozhong
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Peoples R China
推荐引用方式 GB/T 7714	Yue, Xin,Huan, Pin,Hu, Yonghua,et al. Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus[J]. SCIENTIFIC REPORTS,2018,8:13.
APA	Yue, Xin,Huan, Pin,Hu, Yonghua,&Liu, Baozhong.(2018).Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus.SCIENTIFIC REPORTS,8,13.
MLA	Yue, Xin,et al."Integrated transcriptomic and proteomic analyses reveal potential mechanisms linking thermal stress and depressed disease resistance in the turbot Scophthalmus maximus".SCIENTIFIC REPORTS 8(2018):13.