Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians | |
Wang, M.1,3; Wang, B.1; Liu, M.1; Jiang, K.1; Wang, L.1,2 | |
刊名 | ISJ-INVERTEBRATE SURVIVAL JOURNAL |
2018 | |
卷号 | 15页码:19-30 |
关键词 | Argopecten irradians glutathione S-transferase innate immunity |
ISSN号 | 1824-307X |
通讯作者 | Wang, L.(wanglei@qdio.ac.cn) |
英文摘要 | Glutathione S-transferases (GSTs) are a class of enzymes that facilitate the detoxification of xenobiotics and also play important roles in innate immunity. In the present study, a novel sigma class GST gene (designated as AiGST sigma) was cloned from the bay scallop Argopecten irradians via rapid amplification of cDNA ends (RACE) technique. The complete cDNA sequence of AiGSTs consisted of a 5' untranslated regions (UTR) of 48 bp, a 3' UTR of 113 bp with a poly A tail and an open reading frame (ORF) of 618 bp. The ORF encoded a polypeptide of 205 amino acid residues with a calculated molecular mass of approximately 23.11 kDa and a theoretical isoelectric point of 5.354. The deduced amino acid sequence of AiGSTs contained a GST_N domain and a GST_C domain, and exhibited high similarity with other reported sigma class GSTs. In the phylogenetic tree, AiGSTs was located in the sigma class GSTs sub-branch. The AiGSTs mRNA transcripts were constitutively expressed in the tissues of hemocytes, muscle, mantle, gill, hepatopancreas and gonad, with the highest expression level in hemocytes, and the mRNA expression levels of AiGSTs were significantly up-regulated in hemocytes after various pathogen associated molecular patterns (PAMPs) stimulation. The purified recombinant AiGSTs protein exhibited catalytic activity against the common substrate 1-chloro-2, 4-dinitrobenzene (CDNB) with low thermal stability and narrow optimum pH spectrum. All these results indicated that AiGSTs was a fragile but efficient antioxidant enzyme and was potentially involved in the innate immune responses of scallop. |
资助项目 | Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] |
WOS研究方向 | Immunology ; Zoology |
语种 | 英语 |
出版者 | INVERTEBRATE SURVIVAL JOURNAL |
WOS记录号 | WOS:000423724700004 |
内容类型 | 期刊论文 |
源URL | [http://ir.qdio.ac.cn/handle/337002/157992] |
专题 | 海洋研究所_实验海洋生物学重点实验室 |
通讯作者 | Wang, L. |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Res Platform Marine Mol Biotechnol, Qingdao 266237, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, M.,Wang, B.,Liu, M.,et al. Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:19-30. |
APA | Wang, M.,Wang, B.,Liu, M.,Jiang, K.,&Wang, L..(2018).Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians.ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,19-30. |
MLA | Wang, M.,et al."Sequence features, expression profiles and biochemical characteristics of a sigma class glutathione S-transferase gene (AiGST sigma) from bay scallop Argopecten irradians".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):19-30. |
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