Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis

doi:10.1016/j.dci.2017.12.028

CORC > 海洋研究所 > 中国科学院海洋研究所

	Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis
	Li, Yi-qun 1,2,3; Sun, Li 1,2; Li, Jun 4,5
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2018-05-01
卷号	82 页码:31-38
关键词	Scophthalmus maximus IgM(+) cells Endocytosis Macropinocytosis
ISSN号	0145-305X
DOI	10.1016/j.dci.2017.12.028
通讯作者	Sun, Li(lsun@qdio.ac.cn) ; Li, Jun(jli@lssu.edu)
英文摘要	Increasing evidence has demonstrated support for the endocytic capacities of teleost B cells. In the present study, the ability of turbot IgM(+) B cells to ingest microspheres of different sizes and the corresponding internalization pathways were investigated. The results showed that IgM(+) B cells exhibited relatively high endocytic capacities for 0.5 mu m and 1 mu m latex beads, and that different mechanisms were employed for IgM(+) and IgM(-) cells to uptake 0.5 gm and 1 gm beads. For 0.5 gm beads, IgM(+) B cells apparently employed macropinocytosis-dependent endocytic pathway, whereas IgM(-) cells utilized a different process involving both clathrin-and caveolae-mediated pathways. For the uptake of 1 mu m beads, IgM(+) cells relied mainly on macropinocytosis and partially on caveolae-mediated pathway, while IgM(-) cells utilized the routes similar to that of internalizing 0.5 mu m beads. Consistently, the internalized microspheres were co-localized with high-molecular-mass dextran in IgM(+) phagocytic cells. In addition to latex beads, IgM(+) B cells could also ingest inactivated bacteria predominately through macropinocytosis and caveolae-mediated endocytosis. These results collectively indicated that macropinocytosis is principally responsible for particle uptake by turbot IgM(+) B cells. (C) 2018 Elsevier Ltd. All rights reserved.
资助项目	National Natural Science Foundation of China[31528019] ; AoShan Talents Program from Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000427668700003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/158341]
专题	中国科学院海洋研究所
通讯作者	Sun, Li; Li, Jun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 5.Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI 49783 USA
推荐引用方式 GB/T 7714	Li, Yi-qun,Sun, Li,Li, Jun. Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,82:31-38.
APA	Li, Yi-qun,Sun, Li,&Li, Jun.(2018).Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,82,31-38.
MLA	Li, Yi-qun,et al."Internalization of large particles by turbot (Scophthalmus maximus) IgM(+) B cells mainly depends on macropinocytosis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 82(2018):31-38.