Crystal structure of the putative cytoplasmic protein STM0279 (Hcp2) from Salmonella typhimurium

doi:10.1107/S2053230X17010512

	Crystal structure of the putative cytoplasmic protein STM0279 (Hcp2) from Salmonella typhimurium
	Dong, YH; Lin, QP; Gao ZQ(高增强); Geng Z(耿直); Zhang H(张衡); Dong YH(董宇辉); Gao, ZQ; Geng, Z; Zhang, H
刊名	ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
	2017
卷号	73 页码:463-468
关键词	type VI secretion system crystal structure haemolysin co-regulated protein hexameric rings T6SS assembly
ISSN号	2053-230X
DOI	10.1107/S2053230X17010512
文献子类	Article
英文摘要	STM0279 is a putative cytoplasmic protein from Salmonella typhimurium and was recently renamed haemolysin co-regulated protein 2 (Hcp2), with the neighbouring STM0276 being Hcp1. Both of them are encoded by the type VI secretion system (T6SS) of the Salmonella pathogenicity island 6 (SPI-6) locus and have high sequence identity. The Hcp proteins may function as a vital component of the T6SS nanotube and as a transporter and chaperone of diverse effectors from the bacterial T6SS. In this study, the crystal structure and the oligomeric state in solution of Hcp2 from S. typhimurium (StHcp2) were investigated. The crystal structure refined to 3.0 angstrom resolution showed that the protein is composed of a beta-barrel domain with extended loops and can form hexameric rings as observed in known Hcp homologues. Mutation of the extended loop was found to partly destabilize the hexameric conformation into monomers or cause the production of inclusion bodies, suggesting it has an important role in hexameric ring formation.
WOS关键词	VI SECRETION SYSTEM ; STRUCTURE REFINEMENT ; CELLS ; TARGETS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Crystallography
语种	英语
WOS记录号	WOS:000406871600004
内容类型	期刊论文
源URL	[http://ir.ihep.ac.cn/handle/311005/285248]
专题	高能物理研究所_多学科研究中心
作者单位	中国科学院高能物理研究所
推荐引用方式 GB/T 7714	Dong, YH,Lin, QP,Gao ZQ,et al. Crystal structure of the putative cytoplasmic protein STM0279 (Hcp2) from Salmonella typhimurium[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,2017,73:463-468.
APA	Dong, YH.,Lin, QP.,高增强.,耿直.,张衡.,...&Zhang, H.(2017).Crystal structure of the putative cytoplasmic protein STM0279 (Hcp2) from Salmonella typhimurium.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS,73,463-468.
MLA	Dong, YH,et al."Crystal structure of the putative cytoplasmic protein STM0279 (Hcp2) from Salmonella typhimurium".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 73(2017):463-468.