Protection against homo and hetero-subtypic infuenza A virus by optimized M2e DNA vaccine

doi:10.1080/22221751.2018.1558962

	Protection against homo and hetero-subtypic infuenza A virus by optimized M2e DNA vaccine
	Chen, Ze 1,2; Chen, Quanjiao 4; Lan, Jiaming 3; Chen, Jie 7; He, Bin 7; Shao, Zhiyong 7; Chen, Jianjun 4; Wang, Huadong 5,6; Yao, Yanfeng 7,8
刊名	EMERGING MICROBES & INFECTIONS
	2019-01-16
卷号	8 期号:1 页码:45-54
关键词	Influenza M2e tPA optimized M2e DNA vaccine Universal influenza vaccine
ISSN号	2222-1751
DOI	10.1080/22221751.2018.1558962
英文摘要	Current influenza vaccines provide hemagglutinin strain-specific protection, but rarely provide cross-protection against divergent strains. It is, therefore, particularly important to develop a universal vaccine against conserved proteins or conserved regions of the virus. In this study, we used N-terminal extracellular region of the influenza virus M2 protein (M2e) as the target antigen and constructed two optimized M2e DNA vaccines (p-tPA-p3M2e and p-p3M2e) with increased antigenic epitope density and enhanced antigen secretion. Both vaccines induced high M2e-specific humoral and cellular immune responses in the vaccinated mice. These two vaccines also conferred protection against a lethal infection of homo-subtypic H1N1 virus, with p-tPA-p3M2e being the most effective. In addition, p-tPA-p3M2e also showed cross-protection against different subtypes of the influenza virus (H9N2, H6N6, and H10N8) at varying rates (80%, 40%, and 20%, respectively). After passive immunization, M2e DNA vaccine-induced antibodies in the sera provided complete protection against homologous virus challenge. An analysis of the mechanism underlying this immunization-mediated protection indicates that M2e-specific IgG and T-cell immune responses may play critical roles in the prevention of infection and viral clearance. Taken together, our results indicate that this optimized M2e DNA vaccine is a promising candidate for the development of a universal, broad-spectrum influenza virus vaccine.
资助项目	Wuhan Youth Science and Technology Plan[2016070204010104] ; China Mega-Project for Infectious Disease[2017ZX10103005-005] ; Hubei Provincial Natural Science Foundation of China[2016CFC743] ; Hubei Key Laboratory of Animal Embryo and Molecular Breeding[KLAEMB-2016-06] ; National Natural Science Foundation of China[31570026] ; "One-Three-Five" Research Program of Wuhan Institute of Virology
WOS关键词	INFLUENZA-A VIRUS ; MVA-BASED VACCINE ; MONOCLONAL-ANTIBODY ; CROSS-PROTECTION ; PROTEIN ; IMMUNOGENICITY ; IMMUNIZATION ; ECTODOMAIN ; CHALLENGE ; EPITOPE
WOS研究方向	Immunology ; Microbiology
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000455807500006
资助机构	Wuhan Youth Science and Technology Plan ; Wuhan Youth Science and Technology Plan ; China Mega-Project for Infectious Disease ; China Mega-Project for Infectious Disease ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Key Laboratory of Animal Embryo and Molecular Breeding ; Hubei Key Laboratory of Animal Embryo and Molecular Breeding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; "One-Three-Five" Research Program of Wuhan Institute of Virology ; "One-Three-Five" Research Program of Wuhan Institute of Virology ; Wuhan Youth Science and Technology Plan ; Wuhan Youth Science and Technology Plan ; China Mega-Project for Infectious Disease ; China Mega-Project for Infectious Disease ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Key Laboratory of Animal Embryo and Molecular Breeding ; Hubei Key Laboratory of Animal Embryo and Molecular Breeding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; "One-Three-Five" Research Program of Wuhan Institute of Virology ; "One-Three-Five" Research Program of Wuhan Institute of Virology ; Wuhan Youth Science and Technology Plan ; Wuhan Youth Science and Technology Plan ; China Mega-Project for Infectious Disease ; China Mega-Project for Infectious Disease ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Key Laboratory of Animal Embryo and Molecular Breeding ; Hubei Key Laboratory of Animal Embryo and Molecular Breeding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; "One-Three-Five" Research Program of Wuhan Institute of Virology ; "One-Three-Five" Research Program of Wuhan Institute of Virology ; Wuhan Youth Science and Technology Plan ; Wuhan Youth Science and Technology Plan ; China Mega-Project for Infectious Disease ; China Mega-Project for Infectious Disease ; Hubei Provincial Natural Science Foundation of China ; Hubei Provincial Natural Science Foundation of China ; Hubei Key Laboratory of Animal Embryo and Molecular Breeding ; Hubei Key Laboratory of Animal Embryo and Molecular Breeding ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; "One-Three-Five" Research Program of Wuhan Institute of Virology ; "One-Three-Five" Research Program of Wuhan Institute of Virology
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/14258]
专题	中国科学院武汉物理与数学研究所
通讯作者	Chen, Jianjun
作者单位	1.Shanghai Inst Biol Prod, Shanghai, Peoples R China 2.Hunan Normal Univ, Coll Life Sci, Changsha, Hunan, Peoples R China 3.Hebei Med Univ, Dept Pathogen Biol, Shijiazhuang, Hebei, Peoples R China 4.Chinese Acad Sci, CAS Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 5.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan, Hubei, Peoples R China 6.Chinese Acad Sci, Wuhan Inst Phys & Math, Ctr Brain Sci, Key Lab Magnet Resonance Biol Syst, Wuhan, Hubei, Peoples R China 7.Wuhan Acad Agr Sci & Technol, Inst Anim Husb & Vet Sci, Wuhan, Hubei, Peoples R China 8.Chinese Acad Sci, Wuhan Inst Virol, Natl Biosafety Lab, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Chen, Ze,Chen, Quanjiao,Lan, Jiaming,et al. Protection against homo and hetero-subtypic infuenza A virus by optimized M2e DNA vaccine[J]. EMERGING MICROBES & INFECTIONS,2019,8(1):45-54.
APA	Chen, Ze.,Chen, Quanjiao.,Lan, Jiaming.,Chen, Jie.,He, Bin.,...&Yao, Yanfeng.(2019).Protection against homo and hetero-subtypic infuenza A virus by optimized M2e DNA vaccine.EMERGING MICROBES & INFECTIONS,8(1),45-54.
MLA	Chen, Ze,et al."Protection against homo and hetero-subtypic infuenza A virus by optimized M2e DNA vaccine".EMERGING MICROBES & INFECTIONS 8.1(2019):45-54.