An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts

doi:10.1038/s41598-018-26620-0

	An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts
	Mevaere, Jimmy 1; Goulard, Christophe 1; Schneider, Olha 2; Sekurova, Olga N.3; Ma, Haiyan 1,5; Zirah, Severine 1; Afonso, Carlos 4; Rebuffat, Sylvie 1; Zotchev, Sergey B.3; Li, Yanyan 1
刊名	SCIENTIFIC REPORTS
	2018-05-29
卷号	8 期号:1 页码:11
ISSN号	2045-2322
DOI	10.1038/s41598-018-26620-0
英文摘要	Lasso peptides are ribosomally synthesized and post-translationally modified peptides produced by bacteria. They are characterized by an unusual lariat-knot structure. Targeted genome scanning revealed a wide diversity of lasso peptides encoded in actinobacterial genomes, but cloning and heterologous expression of these clusters turned out to be problematic. To circumvent this, we developed an orthogonal expression system for heterologous production of actinobacterial lasso peptides in Streptomyces hosts based on a newly-identified regulatory circuit from Actinoalloteichus fjordicus. Six lasso peptide gene clusters, mainly originating from marine Actinobacteria, were chosen for proof-of-concept studies. By varying the Streptomyces expression hosts and a small set of culture conditions, three new lasso peptides were successfully produced and characterized by tandem MS. The newly developed expression system thus sets the stage to uncover and bioengineer the chemodiversity of actinobacterial lasso peptides. Moreover, our data provide some considerations for future bioprospecting efforts for such peptides.
资助项目	European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH[613877] ; University of Vienna ; NTNU ; City of Paris
WOS关键词	BIOSYNTHETIC GENE CLUSTERS ; BACTERIAL RNA-POLYMERASE ; GENOME-MINING APPROACH ; MASS-SPECTROMETRY ; NATURAL-PRODUCTS ; MICROCIN J25 ; BIOLOGICAL-ACTIVITY ; SIAMYCIN I ; NOMENCLATURE ; IONS
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000433290000011
资助机构	European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; 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European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; 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University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; European Commission under the 7th Framework Program through the Collaborative Project action STREPSYNTH ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; University of Vienna ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; NTNU ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris ; City of Paris
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/30428]
专题	水生生物研究所_藻类生物技术和生物能源研发中心_期刊论文
通讯作者	Zotchev, Sergey B.; Li, Yanyan
作者单位	1.Sorbonne Univ, CNRS, Lab Mol Commun & Adaptat Microorganisms, MCAM,UMR 7245,MNHN, CP 54,57 Rue Cuvier, F-75005 Paris, France 2.Norwegian Univ Sci & Technol NTNU, Dept Biotechnol, N-7491 Trondheim, Norway 3.Univ Vienna, Dept Pharmacognosy, Althanstr 14, A-1090 Vienna, Austria 4.Normandie Univ, CNRS, UNIROUEN, INSA Rouen,COBRA, Rouen, France 5.Chinese Acad Sci, Inst Hydrobiol, Ctr Microalgal Biotechnol & Biofuels, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Mevaere, Jimmy,Goulard, Christophe,Schneider, Olha,et al. An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts[J]. SCIENTIFIC REPORTS,2018,8(1):11.
APA	Mevaere, Jimmy.,Goulard, Christophe.,Schneider, Olha.,Sekurova, Olga N..,Ma, Haiyan.,...&Li, Yanyan.(2018).An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts.SCIENTIFIC REPORTS,8(1),11.
MLA	Mevaere, Jimmy,et al."An orthogonal system for heterologous expression of actinobacterial lasso peptides in Streptomyces hosts".SCIENTIFIC REPORTS 8.1(2018):11.