Isolation and characterization of microsatellite markers in a highland fish, <it><bold>Pareuchiloglanis sinensis</it></bold> (Siluriformes: Sisoridae) by next-generation sequencing

doi:10.1007/s12041-018-0997-6

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物多样性与资源保护研究中心 > 期刊论文

	Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing
	Chen, Weitao 1,2; He, Shunping 1
刊名	JOURNAL OF GENETICS
	2018-12-01
卷号	97 页码:111-116
关键词	microsatellite markers Dadu river RNA-seq Pareuchiloglanis sinensis
ISSN号	0022-1333
DOI	10.1007/s12041-018-0997-6
通讯作者	He, Shunping(clad@ihb.ac.cn)
英文摘要	Pareuchiloglanis sinensis (Siluriformes: Sisoridae) is an endemic and highland fish species which occurs only in some rivers of south-west China. In this study, the isolation and characterization of polymorphic microsatellite loci of this fish species by next-generation sequencing is described. A total of 9471 simple-sequence repeats (SSRs) were observed from RNA-seq data. One hundred and twenty primer pairs were chosen randomly and validated across 48 P. sinensis individuals collected from the Dadu river (a branch of the Yangtze river) of which 28 polymorphic microsatellite loci were detected. The number of alleles ranged from 2 to 14, with an average of seven alleles per locus. Twenty loci exhibited high polymorphism with the polymorphism information content (PIC) larger than 0.5. The mean observed and expected heterozygosity varied from 0.104 to 0.958 and 0.157 to 0.844, with an average of 0.583 and 0.613, respectively. The microsatellite markers characterized in the current study serve as a useful tool for the conversation genetic studies and population evaluation of P. sinensis.
资助项目	National Natural Science Foundation of China[31130049] ; National Natural Science Foundation of China[U1036603]
WOS研究方向	Genetics & Heredity
语种	英语
出版者	INDIAN ACAD SCIENCES
WOS记录号	WOS:000451438500014
资助机构	National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/28985]
专题	水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文
通讯作者	He, Shunping
作者单位	1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.CAFS, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Chen, Weitao,He, Shunping. Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing[J]. JOURNAL OF GENETICS,2018,97:111-116.
APA	Chen, Weitao,&He, Shunping.(2018).Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing.JOURNAL OF GENETICS,97,111-116.
MLA	Chen, Weitao,et al."Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing".JOURNAL OF GENETICS 97(2018):111-116.