Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe

doi:10.1093/nar/gky665

CORC > 化学研究所 > 中国科学院化学研究所 > 分子动态与稳态结构实验室

	Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe
	Zhang, Suge 1,2; Sun, Hongxia 1; Wang, Lixia 1; Liu, Yan 1; Chen, Hongbo 1,2; Li, Qian 1; Guan, Aijiao 1; Liu, Meirong 3; Tang, Yalin 1,2
刊名	NUCLEIC ACIDS RESEARCH
	2018-09-06
卷号	46 期号:15 页码:7522-7532
ISSN号	0305-1048
DOI	10.1093/nar/gky665
英文摘要	G-quadruplex DNA has been viewed as a prospective anti-cancer target owing to its potential biological relevance. Real-time monitoring of DNA G-quadruplex structures in living cells can provide valuable insights into the relationship between G-quadruplex formation and its cellular consequences. However, the probes capable of detecting DNA G-quadruplexes in living cells are still very limited. Herein, we reported a new fluorescent probe, IMT, for real-time visualization of DNA G-quadruplex structures in living cells. Using IMT as a fluorescent indicator, the quantity changes of DNA G-quadruplex at different points in time during continuous cellular progression responding to Aphidicolin and Hydroxyurea treatment have been directly visualized. Our data demonstrate that IMT will be a valuable tool for exploring DNA G-quadruplexes in live cells. Further application of IMT in fluorescence imaging may reveal more information on the roles of DNA G-quadruplexes in biological systems.
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000444148100011
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/41890]
专题	化学研究所_分子动态与稳态结构实验室
通讯作者	Sun, Hongxia; Tang, Yalin
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, CAS Res Educ Ctr Excellence Mol Sci,Inst Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Ctr Physiochem Anal & Measurement, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Suge,Sun, Hongxia,Wang, Lixia,et al. Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe[J]. NUCLEIC ACIDS RESEARCH,2018,46(15):7522-7532.
APA	Zhang, Suge.,Sun, Hongxia.,Wang, Lixia.,Liu, Yan.,Chen, Hongbo.,...&Tang, Yalin.(2018).Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe.NUCLEIC ACIDS RESEARCH,46(15),7522-7532.
MLA	Zhang, Suge,et al."Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe".NUCLEIC ACIDS RESEARCH 46.15(2018):7522-7532.