Poly-DL-lactide-poly (ethylene glycol) microspheres as oral and parenteral delivery systems for hepatitis B surface antigen

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	Poly-DL-lactide-poly (ethylene glycol) microspheres as oral and parenteral delivery systems for hepatitis B surface antigen
	Li, XH ; Zhou, SB ; Wu, XR ; Yuan, ML ; Liu, L ; Jia, WX ; Deng, XM ; Huang, ZT
刊名	JOURNAL OF APPLIED POLYMER SCIENCE
	2002-01-24
卷号	83 期号:4 页码:850-856
关键词	Biodegradable Drug Delivery Systems Polyester Microencapsulation Protein
ISSN号	0021-8995
英文摘要	This project was aimed at illustrating the potential Use of poly-DL-lactide-poly(ethylene glycol) (PELA) microspheres as a hepatitis B surface antigen (HBsAg) delivery system following subcutaneous (s.c.) or oral immunization over the current injection of an alum-absorbed antigen. The antigen-loading microspheres were elaborated by the solvent-extraction method based on the formation of modified multiple w/o/w, emulsion. The microspheres were characterized by their particle size, HBsAg entrapment, and in vitro HBsAg release behavior. Balb/c mice were immunized with an s.c. injection and oral administration of a single dose and two doses of a microsphere formulation. For comparison, the alum-absorbed HBsAg-immunized mice had a following intramuscular (i.m.) injection at weeks 0 and 4. At weeks 8, 10, 14, and 24 postadministration, the blood and saliva samples were collected and detected by the enzyme-linked immunosorbed assay (ELISA) method. A single injection of HBsAg/PELA microspheres could induce a serum IgG antibody level comparable to the two-injection alum-absorbed HBsAg at the 14th week and higher than that at the 24th week. The saliva IgA of peroral groups was significantly higher than that of the s.c. injection of a microsphere formulation and i.m. injection of soluble antigen. These preliminary results demonstrated the potential of oral administration of antigen-loading microspheres in the induction of a secretary immune response, and it is suggested that a single-dose s.c. injection of antigen-loading microspheres would be an efficient immunization schedule to elicit a protective response. (C) 2002 John Wiley & Sons, Inc. J Appl Polym Sci 83: 850-856, 2002.
语种	英语
出版者	JOHN WILEY & SONS INC
WOS记录号	WOS:000172426900016
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/79421]
专题	中国科学院化学研究所
通讯作者	Li, XH
作者单位	1.Chinese Acad Sci, Chengdu Inst Organ Chem, Chengdu 610041, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 3.W China Univ Med Sci, Chengdu 610041, Peoples R China
推荐引用方式 GB/T 7714	Li, XH,Zhou, SB,Wu, XR,et al. Poly-DL-lactide-poly (ethylene glycol) microspheres as oral and parenteral delivery systems for hepatitis B surface antigen[J]. JOURNAL OF APPLIED POLYMER SCIENCE,2002,83(4):850-856.
APA	Li, XH.,Zhou, SB.,Wu, XR.,Yuan, ML.,Liu, L.,...&Huang, ZT.(2002).Poly-DL-lactide-poly (ethylene glycol) microspheres as oral and parenteral delivery systems for hepatitis B surface antigen.JOURNAL OF APPLIED POLYMER SCIENCE,83(4),850-856.
MLA	Li, XH,et al."Poly-DL-lactide-poly (ethylene glycol) microspheres as oral and parenteral delivery systems for hepatitis B surface antigen".JOURNAL OF APPLIED POLYMER SCIENCE 83.4(2002):850-856.