A transformer of molecular beacon for sensitive and real-time detection of phosphatases with effective inhibition of the false positive signals

doi:10.1016/j.bios.2011.06.049

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	A transformer of molecular beacon for sensitive and real-time detection of phosphatases with effective inhibition of the false positive signals
	Zhang, Chen; Su, Xin; Liang, Yuan; Zhu, Xiaocui; Song, Chen; Zhao, Meiping
刊名	BIOSENSORS & BIOELECTRONICS
	2011-10-15
卷号	28 期号:1 页码:13-16
关键词	Molecular Beacon Phosphatase Activity Real-time Detection Inhibition Of False-positive Signals
ISSN号	0956-5663
DOI	10.1016/j.bios.2011.06.049
英文摘要	Molecular beacons (MBs) have shown great potential in measurement of enzyme activities. However, currently available methods for monitoring of phosphatases only use MBs as a signal reporter. Extra substrates for the phosphatases are needed to hybridize to the MB either as a primer or as a template. Moreover, few MB-based methods have been used to detect enzyme activities in real biological samples due to insufficient sensitivity or false positive interference signals caused by nonspecific nucleases. In this work, a novel type of fluorescent probe was designed and synthesized for monitoring of phosphatases by integrating the DNA substrate and the signaling structures into a single molecule. Such a new design not only significantly simplified the probing system and greatly enhanced the sensitivity, but also offered a practical way to guard against the false-positive signal problems in the application to real samples. The unique design of the assay format should be widely applicable to many other enzymatic assays using oligonucleotide fluorescent probes. (C) 2011 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000295661700002
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/73709]
专题	中国科学院化学研究所
通讯作者	Zhao, Meiping
作者单位	Peking Univ, Beijing Natl Lab Mol Sci, MOE Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Chen,Su, Xin,Liang, Yuan,et al. A transformer of molecular beacon for sensitive and real-time detection of phosphatases with effective inhibition of the false positive signals[J]. BIOSENSORS & BIOELECTRONICS,2011,28(1):13-16.
APA	Zhang, Chen,Su, Xin,Liang, Yuan,Zhu, Xiaocui,Song, Chen,&Zhao, Meiping.(2011).A transformer of molecular beacon for sensitive and real-time detection of phosphatases with effective inhibition of the false positive signals.BIOSENSORS & BIOELECTRONICS,28(1),13-16.
MLA	Zhang, Chen,et al."A transformer of molecular beacon for sensitive and real-time detection of phosphatases with effective inhibition of the false positive signals".BIOSENSORS & BIOELECTRONICS 28.1(2011):13-16.