Fluorescent detection of singlet oxygen: Amplifying signal transduction and improving sensitivity based on intramolecular FRET of anthryl appended porphyrins

doi:10.1007/s11434-011-4736-0

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	Fluorescent detection of singlet oxygen: Amplifying signal transduction and improving sensitivity based on intramolecular FRET of anthryl appended porphyrins
	You MingXu 1; Wang YongXiang 1; Wang Hao 1; Yang RongHua 1,2
刊名	CHINESE SCIENCE BULLETIN
	2011-11-01
卷号	56 期号:31 页码:3253-3259
关键词	(1)o(2) Anthracene Porphyrin Fluorescence Probe Fret
ISSN号	1001-6538
DOI	10.1007/s11434-011-4736-0
英文摘要	Two new anthryl-appended porphyrin dyads have been synthesized and used as highly selective and sensitive fluorescence probes for singlet oxygen ((1)O(2)). The design strategy for the probes is directed by the idea of intramolecular fluorescence resonance energy transfer (FRET) interactions and carried out by incorporation of an electron-rich fluorophore (porphyrin) with a reactive anthracene for (1)O(2). The molecular recognition is based on the specific interaction of (1)O(2) with the inner anthracene moiety, and the signal reporter for the recognition process is the porphyrin fluorescence. As a result of overlap of the emission band of the anthracene with the absorbance band of the porphyrin, intramolecular FRET occurs between the anthracene (donor) and the porphyrin (acceptor). The effective light absorbed by the porphyrin and, concomitantly, the emitted light intensity are thus modulated by the emission intensity of the anthracene. Upon reaction with reactive oxygen species such as hydrogen peroxide, hypochlorite, superoxide, hydroxyl radicals, and (1)O(2), the probes exhibit a selective response toward (1)O(2). In addition, significant amplification of the signal transducer is observed. The feasibility of the design was demonstrated by monitoring the (1)O(2) generated from a MoO(4)(2-)/H(2)O(2) system. The results clearly demonstrate that the synthesized probes exhibit both high selectivity and high sensitivity for (1)O(2). The fluorescence reaction and signal amplification mechanism of the system were both discussed, clearly confirming that the introduction of electron-rich porphyrin units into the 9,10-positions of anthracene can improve the response sensitivity and activate the probe's reactivity toward (1)O(2).
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000296640800005
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/73183]
专题	中国科学院化学研究所
通讯作者	Yang RongHua
作者单位	1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Ctr Biomed Engn, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China
推荐引用方式 GB/T 7714	You MingXu,Wang YongXiang,Wang Hao,et al. Fluorescent detection of singlet oxygen: Amplifying signal transduction and improving sensitivity based on intramolecular FRET of anthryl appended porphyrins[J]. CHINESE SCIENCE BULLETIN,2011,56(31):3253-3259.
APA	You MingXu,Wang YongXiang,Wang Hao,&Yang RongHua.(2011).Fluorescent detection of singlet oxygen: Amplifying signal transduction and improving sensitivity based on intramolecular FRET of anthryl appended porphyrins.CHINESE SCIENCE BULLETIN,56(31),3253-3259.
MLA	You MingXu,et al."Fluorescent detection of singlet oxygen: Amplifying signal transduction and improving sensitivity based on intramolecular FRET of anthryl appended porphyrins".CHINESE SCIENCE BULLETIN 56.31(2011):3253-3259.