Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides

doi:10.1002/cbic.201100031

CORC > 化学研究所 > 中国科学院化学研究所

	Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides
	Huang, Yanyan 1; Zhao, Rui 1; Fu, Yabin 2; Zhang, Qundan 1; Xiong, Shaoxiang 1; Li, Li 2; Zhou, Rouli 2; Liu, Guoquan 1; Chen, Yi 1
刊名	CHEMBIOCHEM
	2011-05-16
卷号	12 期号:8 页码:1209-1215
关键词	Affinity Chromatography Cancer Cell Imaging Molecular Recognition Peptides
ISSN号	1439-4227
DOI	10.1002/cbic.201100031
英文摘要	Specific detection and in vivo tracing of cancer biomarkers are important for cancer analysis. In this work, a simple and effective strategy for developing peptide probes was established. Peptides were rationally designed by using an antisense peptide approach directed towards an extracellular fragment (EL2) of a novel tumor-related protein LAPTM4B. Positional-scanning and stepwise affinity screening was employed to obtain an optimal peptide AP2H (IHGHHIISVG). The dissociation constant between the two small peptides, AP2H and the target EL2, was 5.51 mu m under physiological conditions. Fluorescence imaging assays indicated that AP2H can recognize live hepatoma cells by targeting the LAPTM4B protein on the cell surface with high specificity, low cytotoxicity and desirable cell penetrability. Compared to negative control cells, AP2H could differentiate cells with different expression levels of LAPTM4B. The screened peptide probe for molecular signatures of cancer cells, based on targeting the LAPTM4B protein, has potential applications in cancer diagnosis and targetable drug delivery.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000290862400011
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/72813]
专题	中国科学院化学研究所
通讯作者	Zhao, Rui
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, CAS Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China 2.Peking Univ, Dept Cell Biol, Sch Basic Med Sci, Beijing 100191, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yanyan,Zhao, Rui,Fu, Yabin,et al. Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides[J]. CHEMBIOCHEM,2011,12(8):1209-1215.
APA	Huang, Yanyan.,Zhao, Rui.,Fu, Yabin.,Zhang, Qundan.,Xiong, Shaoxiang.,...&Chen, Yi.(2011).Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides.CHEMBIOCHEM,12(8),1209-1215.
MLA	Huang, Yanyan,et al."Highly Specific Targeting and Imaging of Live Cancer Cells by Using a Peptide Probe Developed from Rationally Designed Peptides".CHEMBIOCHEM 12.8(2011):1209-1215.