Fluorescence staining and confocal laser scanning microscopy study of hydrogen-bonded poly(vinylpyrrolidone)/poly(acrylic acid) film

doi:10.1016/j.colsurfa.2011.09.037

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	Fluorescence staining and confocal laser scanning microscopy study of hydrogen-bonded poly(vinylpyrrolidone)/poly(acrylic acid) film
	Cao, Yugai 1,2; Wang, Chunyan 1,2; Yang, Shuguang 1,2; Li, Yuefang 3; Yang, Xiaoli 1,2; Zhang, Chenggui 3; Ma, Jinghong 1,2; Xu, Jian 1,2,3
刊名	COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
	2011-12-05
卷号	392 期号:1 页码:83-87
关键词	Layer-by-layer Assembly Hydrogen Bonding Fluorescence Confocal Laser Scanning Microscopy
ISSN号	0927-7757
DOI	10.1016/j.colsurfa.2011.09.037
英文摘要	Poly(vinylpyrrolidone) (PVPON) and poly(acrylic acid) (PAA) were layer-by-layer (LBL) assembled to prepare the thin films based on hydrogen-bonding complexation. The hydrogen-bonded PVPON/PAA films were incubated in acidic, neutral and basic vapors separately. To study the morphologies after incubation, the films were stained by pH-sensitive fluorescent dyes using chemical and physical ways, and investigated with confocal laser scanning microscope (CLSM). The chemical way (labeling) was covalently linking fluoresceinamine (FAM) to some monomer units of PAA while the physical way was adsorbing rhodamine B (RB) molecules from dilute solution. Atomic force microscope (AFM) was combined with CLSM to find that after incubation in neutral or basic vapor the hydrogen-bonded PVPON/PAA films form porous structure and the pores are through the whole film. (C) 2011 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000298899300010
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/72011]
专题	中国科学院化学研究所
通讯作者	Yang, Shuguang
作者单位	1.Donghua Univ, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201620, Peoples R China 2.Donghua Univ, Coll Mat Sci & Engn, Shanghai 201620, Peoples R China 3.Chinese Acad Sci, Lab Polymer Phys & Chem, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Cao, Yugai,Wang, Chunyan,Yang, Shuguang,et al. Fluorescence staining and confocal laser scanning microscopy study of hydrogen-bonded poly(vinylpyrrolidone)/poly(acrylic acid) film[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2011,392(1):83-87.
APA	Cao, Yugai.,Wang, Chunyan.,Yang, Shuguang.,Li, Yuefang.,Yang, Xiaoli.,...&Xu, Jian.(2011).Fluorescence staining and confocal laser scanning microscopy study of hydrogen-bonded poly(vinylpyrrolidone)/poly(acrylic acid) film.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,392(1),83-87.
MLA	Cao, Yugai,et al."Fluorescence staining and confocal laser scanning microscopy study of hydrogen-bonded poly(vinylpyrrolidone)/poly(acrylic acid) film".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 392.1(2011):83-87.