Carbazole tricationic salt: A novel potential two-photon fluorescent DNA probe for nucleic imaging of cells

doi:10.1007/s11434-010-4176-2

CORC > 化学研究所 > 中国科学院化学研究所

	Carbazole tricationic salt: A novel potential two-photon fluorescent DNA probe for nucleic imaging of cells
	Zhao Ning 1; Zhang YuanHong 1; Liu Xin 1; Yu XiaoQiang 1; Ge MaoFa 2
刊名	CHINESE SCIENCE BULLETIN
	2010-11-01
卷号	55 期号:32 页码:3661-3667
关键词	Two-photon Fluorescence Microscopy Two-photon Fluorescent Probe Dna Titration Dna Staining
ISSN号	1001-6538
DOI	10.1007/s11434-010-4176-2
英文摘要	A new carbazole tricationic salt, 4,4'-(1E,1'E)-2,2'-(9-(2-(1-(2-hydroxyethyl)pyridinium-4-yl)ethyl)-9H-carbazole-3,6-diyl) bis(ethane-2,1-diyl) bis(1-(2-hydroxyethyl)pyridinium) iodide (THEPC) was synthesized. Photophysical experiments have shown that THEPC has large two-photon excited fluorescence action cross-sections (33 GM in the presence of DNA), which ranks THEPC as a good biological fluorophore. The results from electronic absorption, circle dichroism and single-/two-photon fluorescence emission spectra suggest that THEPC can strongly bind to DNA, with an intrinsic binding constant of 5.79 x 10(6) L mol(-1). THEPC has better photostability under one- or two-photon excitation conditions. Finally, the staining photos from two-photon fluorescence microscopy (TPM) show that THEPC can exclusively label the nucleus with high contrast and without image distortion. These remarkable properties and optimized imaging ability make THEPC an attractive DNA probe in TPM.
语种	英语
出版者	SCIENCE CHINA PRESS
WOS记录号	WOS:000285026300006
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/70257]
专题	中国科学院化学研究所
通讯作者	Yu XiaoQiang
作者单位	1.Shandong Univ, State Key Lab Crystal Mat, Jinan 250100, Peoples R China 2.Chinese Acad Sci, Beijing Natl Lab Mol Sci BNLM, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhao Ning,Zhang YuanHong,Liu Xin,et al. Carbazole tricationic salt: A novel potential two-photon fluorescent DNA probe for nucleic imaging of cells[J]. CHINESE SCIENCE BULLETIN,2010,55(32):3661-3667.
APA	Zhao Ning,Zhang YuanHong,Liu Xin,Yu XiaoQiang,&Ge MaoFa.(2010).Carbazole tricationic salt: A novel potential two-photon fluorescent DNA probe for nucleic imaging of cells.CHINESE SCIENCE BULLETIN,55(32),3661-3667.
MLA	Zhao Ning,et al."Carbazole tricationic salt: A novel potential two-photon fluorescent DNA probe for nucleic imaging of cells".CHINESE SCIENCE BULLETIN 55.32(2010):3661-3667.