Direct Measurement of the Rates and Barriers on Forward and Reverse Diffusions of Intramolecular Collision in Overhang Oligonucleotides

doi:10.1021/jp101173y

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	Direct Measurement of the Rates and Barriers on Forward and Reverse Diffusions of Intramolecular Collision in Overhang Oligonucleotides
	Qu, Peng ; Yang, Xinxing ; Li, Xun ; Zhou, Xiaoxue ; Zhao, Xin Sheng 1
刊名	JOURNAL OF PHYSICAL CHEMISTRY B
	2010-06-24
卷号	114 期号:24 页码:8235-8243
ISSN号	1520-6106
DOI	10.1021/jp101173y
英文摘要	The dynamics of end-to-interior (Type I) and end-to-end (Type II) collisions in a dangling overhang anchored on a double-stranded DNA (dsDNA) was studied by monitoring the fluorescence quenching of tetramethylrhodamine (TMR) by guanosine residues through combining photoinduced electron transfer (PET) with fluorescence correlation spectroscopy (FCS) at different temperatures. TMR and guanosine residues are separated by a double helix with dangling bases ranging from 2 to 16. By analyzing the FCS data, we obtained the forward and reverse intrachain diffusion rate constants and respective barriers. For both Type I and Type II collisions, the intrachain diffusion rates followed the scaling law of the Gaussian chain model. Especially, the reverse intrachain diffusion rate was insensitive to the base length of separation. Both the activation enthalpy and activation entropy of the forward and reverse diffusions were length independent. The comparison between Type I and Type II collisions shows that the collision rate of end-to-interior is slower than that of end-to-end. The phenomenon is further checked in detail by a series of dangling DNA with the same separation length but different tail lengths (Type III).
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000278846100024
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/69471]
专题	中国科学院化学研究所
通讯作者	Zhao, Xin Sheng
作者单位	1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Qu, Peng,Yang, Xinxing,Li, Xun,et al. Direct Measurement of the Rates and Barriers on Forward and Reverse Diffusions of Intramolecular Collision in Overhang Oligonucleotides[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2010,114(24):8235-8243.
APA	Qu, Peng,Yang, Xinxing,Li, Xun,Zhou, Xiaoxue,&Zhao, Xin Sheng.(2010).Direct Measurement of the Rates and Barriers on Forward and Reverse Diffusions of Intramolecular Collision in Overhang Oligonucleotides.JOURNAL OF PHYSICAL CHEMISTRY B,114(24),8235-8243.
MLA	Qu, Peng,et al."Direct Measurement of the Rates and Barriers on Forward and Reverse Diffusions of Intramolecular Collision in Overhang Oligonucleotides".JOURNAL OF PHYSICAL CHEMISTRY B 114.24(2010):8235-8243.