A Water-Soluble Conjugated Polymer for Protein Identification and Denaturation Detection

doi:10.1002/asia.201000363

CORC > 化学研究所 > 中国科学院化学研究所

	A Water-Soluble Conjugated Polymer for Protein Identification and Denaturation Detection
	Xu, Qingling; Wu, Chunxian; Zhu, Chunlei; Duan, Xinrui; Liu, Libing; Han, Yuchun; Wang, Yilin; Wang, Shu
刊名	CHEMISTRY-AN ASIAN JOURNAL
	2010
卷号	5 期号:12 页码:2524-2529
关键词	Conjugated Polymers Fluorescence Proteins Sensors
ISSN号	1861-4728
DOI	10.1002/asia.201000363
英文摘要	Rapid and sensitive methods to detect proteins and protein denaturation have become increasingly needful in the field of protcomics, medical diagnostics, and biology. In this paper, we have reported the synthesis of a new cationic water-soluble conjugated polymer that contains fluorene and diene moieties in the backbone (PFDE) for protein identification by sensing an array of PFDE solutions in different ionic strengths using the linear discriminant analysis technique (LDA). The PFDE can form complexes with proteins by electrostatic and/or hydrophobic interactions and exhibits different fluorescence response. Three main factors contribute to the fluorescence response of PFDE, namely, the net charge density on the protein surface, the hydrophobic nature of the protein, and the metallo-protein characteristics. The denaturation of proteins can also be detected using PFDE as a fluorescent probe. The interactions between PFDE and proteins were also studied by dynamic light scattering (DLS) and isothermal titration microcalorimetry (ITC) techniques. In contrast to other methods based on conjugated polymers, the synthesis of a series of quencher or dye-labeled acceptors or protein substrates has been avoided in our method, which significantly reduces the cost and the synthetic complexity. Our method provides promising applications on protein identification and denaturation detection in a simple, fast, and label-free manner based on non-specific interaction-induced perturbation of PFDE fluorescence response.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000285255600012
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/69451]
专题	中国科学院化学研究所
通讯作者	Liu, Libing
作者单位	Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Xu, Qingling,Wu, Chunxian,Zhu, Chunlei,et al. A Water-Soluble Conjugated Polymer for Protein Identification and Denaturation Detection[J]. CHEMISTRY-AN ASIAN JOURNAL,2010,5(12):2524-2529.
APA	Xu, Qingling.,Wu, Chunxian.,Zhu, Chunlei.,Duan, Xinrui.,Liu, Libing.,...&Wang, Shu.(2010).A Water-Soluble Conjugated Polymer for Protein Identification and Denaturation Detection.CHEMISTRY-AN ASIAN JOURNAL,5(12),2524-2529.
MLA	Xu, Qingling,et al."A Water-Soluble Conjugated Polymer for Protein Identification and Denaturation Detection".CHEMISTRY-AN ASIAN JOURNAL 5.12(2010):2524-2529.