High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy

doi:10.1016/j.bpc.2009.09.012

CORC > 化学研究所 > 中国科学院化学研究所

	High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy
	Sang, Min 1,5; Ma, Fei 2; Xie, Jie 3; Chen, Xiao-Bo 4; Wang, Ke-Bin 1; Qin, Xiao-Chun 1; Wang, Wen-Da 1; Zhao, Jing-Quan 3; Li, Liang-Bi 1; Zhang, Jian-Ping 2
刊名	BIOPHYSICAL CHEMISTRY
	2010
卷号	146 期号:1 页码:7-12
关键词	Chlorophyll a Cyt b(6)f Singlet Oxygen Photo-bleaching Epr
ISSN号	0301-4622
DOI	10.1016/j.bpc.2009.09.012
英文摘要	Electron paramagnetic resonance (EPR) spectroscopy was used to detect the light-induced formation of singlet oxygen (O-1(2)center dot) in the intact and the Rieske-depleted cytochrome b(6)f complexes (Cyt b(6)f) from Bryopsis corticulans, as well as in the isolated Rieske Fe-S protein. It is shown that, under white-light illumination and aerobic conditions, chlorophyll a (Chl a) bound in the intact Cyt b(6)f can be bleached by light-induced O-1(2)center dot, and that the 10; production can be promoted by D2O or scavenged by extraneous antioxidants such as L-histidine, ascorbate, beta-carotene and glutathione. Under similar experimental conditions, O-1(2)center dot was also detected in the Rieske-depleted Cyt b(6)f complex, but not in the isolated Rieske Fe-S protein. The results prove that Chl a cofactor, rather than Rieske Fe-S protein, is the specific site of O-1(2)center dot formation, a conclusion which draws further support from the generation of O-1(2)center dot with selective excitation of Chl a using monocolor red light. (C) 2009 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000272440400002
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/69051]
专题	中国科学院化学研究所
通讯作者	Li, Liang-Bi
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 2.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China 3.Chinese Acad Sci, Inst Chem, Key Lab Photochem, Beijing 100080, Peoples R China 4.Hebei Univ Sci & Technol, Sch Biosci & Engn, Shijiazhuang 050018, Hebei Province, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Sang, Min,Ma, Fei,Xie, Jie,et al. High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy[J]. BIOPHYSICAL CHEMISTRY,2010,146(1):7-12.
APA	Sang, Min.,Ma, Fei.,Xie, Jie.,Chen, Xiao-Bo.,Wang, Ke-Bin.,...&Kuang, Ting-Yun.(2010).High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy.BIOPHYSICAL CHEMISTRY,146(1),7-12.
MLA	Sang, Min,et al."High-light induced singlet oxygen formation in cytochrome b(6)f complex from Bryopsis corticulans as detected by EPR spectroscopy".BIOPHYSICAL CHEMISTRY 146.1(2010):7-12.