Effect of functionalized micropatterned PLGA on guided neurite growth

doi:10.1016/j.actbio.2008.09.002

CORC > 化学研究所 > 中国科学院化学研究所

	Effect of functionalized micropatterned PLGA on guided neurite growth
	Yao, Li 1; Wang, Shenguo 4; Cui, Wenjin 4; Sherlock, Richard 2; O'Connell, Claire 2; Damodaran, Gopinath 1; Gorman, Adrienne 3; Windebank, Anthony 5; Pandit, Abhay 1
刊名	ACTA BIOMATERIALIA
	2009-02-01
卷号	5 期号:2 页码:580-588
关键词	Guided Regeneration Micropatterning Laminin Peptide Plga
ISSN号	1742-7061
DOI	10.1016/j.actbio.2008.09.002
英文摘要	When coaptation is not possible in the repair of nerve injuries, a bridge of biomaterial scaffold provides a structural support for neuronal cell growth and guides nerve regeneration. Poly(lactide-co-glycolide) (PLGA) scaffolds have been widely investigated for neural tissue engineering applications. In order to investigate guided neurite growth, we have fabricated micropatterns on PLGA films using laser ablation methods. The micropatterned PLGA films were coated with collagen type I or laminin peptide (PPFLMLLKGSTR) to promote axon growth. Micropatterned PLGA films provide a guidance effect on both early stage neurite outgrowth and elongation. Small (5 mu m) grooves showed more statistically significant parallel neurite growth compared with larger size grooves (10 mu m). Micropatterned PLGA films coated with laminin peptide showed more parallel neurite growth compared with those coated with collagen type I. Primary neurite number and total neurite length per cell decreased oil micropatterned PLGA films compared with the controls. Neurites showed a preference for growth in the microgrooves rather than on the spaces. This study indicates that surface micropatterned structures with conjugated functional molecules can be used to guide neurite growth. (c) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000262684600006
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/67823]
专题	中国科学院化学研究所
通讯作者	Pandit, Abhay
作者单位	1.Natl Univ Ireland, Natl Ctr Biomed Engn Sci, Galway, Ireland 2.Natl Univ Ireland, Natl Ctr Laser Applicat, Galway, Ireland 3.Natl Univ Ireland, Dept Biochem, Galway, Ireland 4.Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, BNLMS, Beijing 100080, Peoples R China 5.Mayo Clin, Dept Neurol, Rochester, MN USA
推荐引用方式 GB/T 7714	Yao, Li,Wang, Shenguo,Cui, Wenjin,et al. Effect of functionalized micropatterned PLGA on guided neurite growth[J]. ACTA BIOMATERIALIA,2009,5(2):580-588.
APA	Yao, Li.,Wang, Shenguo.,Cui, Wenjin.,Sherlock, Richard.,O'Connell, Claire.,...&Pandit, Abhay.(2009).Effect of functionalized micropatterned PLGA on guided neurite growth.ACTA BIOMATERIALIA,5(2),580-588.
MLA	Yao, Li,et al."Effect of functionalized micropatterned PLGA on guided neurite growth".ACTA BIOMATERIALIA 5.2(2009):580-588.