Carbon nanotube-quenched fluorescent oligonucleotides: Probes that fluoresce upon hybridization

doi:10.1021/ja800604z

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	Carbon nanotube-quenched fluorescent oligonucleotides: Probes that fluoresce upon hybridization
	Yang, Ronghua 1,2,3; Jin, Jianyu 1; Chen, Yan 2,3; Shao, Na 1; Kang, Huaizhi 2,3; Xiao, Zeyu 2,3; Tang, Zhiwen 2,3; Wu, Yanrong 2,3; Zhu, Zhi 2,3; Tan, Weihong 2,3
刊名	JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
	2008-07-02
卷号	130 期号:26 页码:8351-8358
ISSN号	0002-7863
DOI	10.1021/ja800604z
英文摘要	We report an effective, novel self-assembled single-wall carbon nanotube (SWNT) complex with an oligonucleotide and demonstrate its feasibility in recognizing and detecting specific DNA sequences in a single step in a homogeneous solution. The key component of this complex is the hairpin-structured fluorescent oligonucleotide that allows the SWNT to function as both a "nanoscaffold" for the oligonucleotide and a "nanoquencher" of the fluorophore. Given this functionality, this carbon nanotube complex represents a new class of universal fluorescence quenchers that are substantially different from organic quenchers and should therefore have many applications in molecular engineering and biosensor development. Competitive binding of a DNA target and SWNTs with the oligonucleotide results in fluorescence signal increments relative to the fluorescence without a target as well as in marked fluorescence quenching. In contrast to the common loop-and-stem configuration of molecular beacons (MBs), this novel fluorescent oligonucleotide needs only one labeled fluorophore, yet the emission can be measured with little or no background interference. This property greatly improves the signal-to-background ratio compared with those for conventional MBs, while the DNA-binding specificity is still maintained by the MB. To test the interaction mechanisms of the fluorescent oligonucleotide with SWNTs and target DNA, thermodynamic analysis and fluorescence anisotropy measurements, respectively, were applied. Our results show that MB/SWNT probes can be an excellent platform for nucleic acid studies and molecular sensing.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000257152800051
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/65555]
专题	中国科学院化学研究所
通讯作者	Yang, Ronghua
作者单位	1.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Univ Florida, Dept Chem, Ctr Res Bionano Interface, Gainesville, FL 32611 USA 3.Univ Florida, Dept Physiol & Funct Genom, Shands Canc Ctr, UF Genet Inst,McKnight Brain Inst, Gainesville, FL 32611 USA
推荐引用方式 GB/T 7714	Yang, Ronghua,Jin, Jianyu,Chen, Yan,et al. Carbon nanotube-quenched fluorescent oligonucleotides: Probes that fluoresce upon hybridization[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2008,130(26):8351-8358.
APA	Yang, Ronghua.,Jin, Jianyu.,Chen, Yan.,Shao, Na.,Kang, Huaizhi.,...&Tan, Weihong.(2008).Carbon nanotube-quenched fluorescent oligonucleotides: Probes that fluoresce upon hybridization.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,130(26),8351-8358.
MLA	Yang, Ronghua,et al."Carbon nanotube-quenched fluorescent oligonucleotides: Probes that fluoresce upon hybridization".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 130.26(2008):8351-8358.