Aptamer-based affinity chromatographic assays for thrombin

doi:10.1021/ac801206s

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	Aptamer-based affinity chromatographic assays for thrombin
	Zhao, Qiang 1; Li, Xing-Fang 1; Shao, Yuanhua 2; Le, X. Chris 1,2
刊名	ANALYTICAL CHEMISTRY
	2008-10-01
卷号	80 期号:19 页码:7586-7593
ISSN号	0003-2700
DOI	10.1021/ac801206s
英文摘要	Affinity chromatographic assays for thrombin were developed using two aptamers as affinity ligands. The efficient capture and step elution of thrombin with NaClO4 enabled the determination of thrombin by using either absorbance or fluorescence detection. Preconcentration of thrombin on the affinity column improved the detection limit of thrombin to 0.1 nM. Using an aptamer for the fibrinogenbinding site of thrombin and a second aptamer for the heparin-binding site, a sandwich chromatographic assay was developed, showing improved selectivity of thrombin detection and eliminating the need for labeling thrombin in the sample. The increased local concentration of aptamers immobilized on monolithic columns favored the formation of aptamer-thrombin complexes, resulting in improved retention and detection of thrombin at trace levels.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000259603100057
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/65107]
专题	中国科学院化学研究所
通讯作者	Le, X. Chris
作者单位	1.Univ Alberta, Dept Lab Med & Pathol, Div Analyt & Environm Toxicol, Edmonton, AB T6G 2G3, Canada 2.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Qiang,Li, Xing-Fang,Shao, Yuanhua,et al. Aptamer-based affinity chromatographic assays for thrombin[J]. ANALYTICAL CHEMISTRY,2008,80(19):7586-7593.
APA	Zhao, Qiang,Li, Xing-Fang,Shao, Yuanhua,&Le, X. Chris.(2008).Aptamer-based affinity chromatographic assays for thrombin.ANALYTICAL CHEMISTRY,80(19),7586-7593.
MLA	Zhao, Qiang,et al."Aptamer-based affinity chromatographic assays for thrombin".ANALYTICAL CHEMISTRY 80.19(2008):7586-7593.