Decompaction of Cationic Gemini Surfactant-Induced DNA Condensates by beta-Cyclodextrin or Anionic Surfactant

doi:10.1021/jp803244f

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	Decompaction of Cationic Gemini Surfactant-Induced DNA Condensates by beta-Cyclodextrin or Anionic Surfactant
	Cao, Meiwen 1; Deng, Manli 1; Wang, Xiao-Ling 2; Wang, Yilin 1
刊名	JOURNAL OF PHYSICAL CHEMISTRY B
	2008-10-30
卷号	112 期号:43 页码:13648-13654
ISSN号	1520-6106
DOI	10.1021/jp803244f
英文摘要	Compaction of DNA by cationic gemini surfactant hexamethylene-1,6-bis-(dodecyldimethylammoniumbromide) (C12C6C12Br2) and the subsequent decompaction of the DNA-C12C6C12Br2 complexes by beta-cyclodextrin (beta-CD) or sodium dodecyl sulfate (SDS) have been studied by using potential and particle size measurements, atomic force microscopy (AFM), isothermal titration microcalorimetry (ITC), and circular dichroism. The results show that C12C6C12Br2 can induce the collapse of DNA into densely packed bead-like structures with smaller size in an all-or-none manner, accompanied by the increase of potential from highly negative values to highly positive values. In the decompaction of the DNA-C12C6C12Br2 complexes, beta-CD and SDS exhibit different behaviors. For beta-CD, the experimental results suggest that it can remove the outlayer hydrophobically bound C12C6C12Br2 Molecules from the DNA-C12C6C12Br2 complexes by inclusion interaction, and the excess beta-CD may attach on the complexes by forming inclusion complexes with the hydrocarbon chains of the electrostatically bound C12C6C12Br2 that cannot be removed. The increase of steric hindrance due to the attachment of beta-CD molecules results in the decompaction of the DNA condensates though the true release of DNA cannot be attained. However, for SDS, the experimental results suggest that it can realize the decompaction and release of DNA from its complexes with C12C6C12Br2 due to both ion-pairing and hydrophobic interaction between SDS and C12C6C12Br2.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000260357700024
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/65017]
专题	中国科学院化学研究所
通讯作者	Wang, Yilin
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Colloid & Interface Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Cao, Meiwen,Deng, Manli,Wang, Xiao-Ling,et al. Decompaction of Cationic Gemini Surfactant-Induced DNA Condensates by beta-Cyclodextrin or Anionic Surfactant[J]. JOURNAL OF PHYSICAL CHEMISTRY B,2008,112(43):13648-13654.
APA	Cao, Meiwen,Deng, Manli,Wang, Xiao-Ling,&Wang, Yilin.(2008).Decompaction of Cationic Gemini Surfactant-Induced DNA Condensates by beta-Cyclodextrin or Anionic Surfactant.JOURNAL OF PHYSICAL CHEMISTRY B,112(43),13648-13654.
MLA	Cao, Meiwen,et al."Decompaction of Cationic Gemini Surfactant-Induced DNA Condensates by beta-Cyclodextrin or Anionic Surfactant".JOURNAL OF PHYSICAL CHEMISTRY B 112.43(2008):13648-13654.