Preparation and Evaluation of a Radioisotope-Incorporated Iron Oxide Core/Au Shell Nanoplatform for Dual Modality Imaging

doi:10.1166/jbn.2008.013

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	Preparation and Evaluation of a Radioisotope-Incorporated Iron Oxide Core/Au Shell Nanoplatform for Dual Modality Imaging
	Zhou, You-Fu 1; Gulaka, Praveen 2; Zhou, Jian 3; Xiao, Ming 1,4; Xu, Dongsheng 4; Hsieh, Jer-Tsong 3; Kodibagkar, Vikram D.1; Sun, Xiankai 1
刊名	Journal of Biomedical Nanotechnology
	2008-12-01
卷号	4 期号:4 页码:474-481
关键词	Mri Nuclear Imaging Prostate Cancer Cell Trafficking Superparamagnetic Iron Oxide Nanoparticles (Spio) Gold Nanoparticles
ISSN号	1550-7033
DOI	10.1166/jbn.2008.013
英文摘要	Magnetic gold-coated iron oxide nanoparticles (Fe@Au NPs) have recently emerged as a new type of iron oxide nanoparticle based magnetic resonance imaging (MRI) contrast agents, in which the gold shell provides a conveniently tunable surface for the presentation of multiple functional molecules. Given the versatility of this nanoplatform and the intrinsic sensitivity limitation of MRI contrast agents, a new approach was developed in this work to incorporate radioisotopes with suitable half-lives into the iron oxide core of Fe@Au NPs and impart the superior sensitivity of nuclear imaging to the nanoplatform. The incorporation of (67)Ga was successfully accomplished by co-precipitation of (67)Gal(3+) with Fe(3+)/Fe(2+) ions at a pH ranging from 4-10. The gold coating procedure was carried out by an iterative hydroxylamine seeding process. Upon the gold deposition, the hydrodynamic radius of the nanoparticles was changed from 23.2 +/- 2.2 nm to 31.7 +/- 2.3 nm, indicating an 8-nm thickness for the gold shell. The Fe@Au NPs were functionalized by lipoic acid (LA) and further conjugated with a polyarginine cell permeation peptide, NH(2)GR11. All the Fe@Au NPs stayed nearly 100% intact in either PBS or rat serum within 72 h. Cell labeling with the LA-modified Fe@Au NPs and NH(2)GR11-conjugated Fe@Au NPs was conducted by using a human prostate cancer cell line (PC-3). It was shown that NH(2)GR11 was able to increase the nanoparticle loading to PC-3 cells by 2-3 times. Shown in a pilot dual-modality imaging study, the LA-modified Fe@Au NP labeled cells could be visualized by both MRI and autoradiography imaging if the labeled PC-3 cell concentrations were above 1 x 10(5) cells/mL. The cell permeation peptide, NH(2)GR11, could significantly enhance the dual-modality detection sensitivity of the nanoplatform labeled PC-3 cells.
语种	英语
出版者	AMER SCIENTIFIC PUBLISHERS
WOS记录号	WOS:000262544200013
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/63105]
专题	中国科学院化学研究所
通讯作者	Sun, Xiankai
作者单位	1.Univ Texas SW Med Ctr Dallas, Dept Radiol, Dallas, TX 75390 USA 2.Univ Texas SW Med Ctr Dallas, Univ Texas Arlington, Biomed Engn Grad Program, Dallas, TX 75390 USA 3.Univ Texas SW Med Ctr Dallas, Dept Urol, Dallas, TX 75390 USA 4.Peking Univ, Coll Chem & Mol Engn, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Zhou, You-Fu,Gulaka, Praveen,Zhou, Jian,et al. Preparation and Evaluation of a Radioisotope-Incorporated Iron Oxide Core/Au Shell Nanoplatform for Dual Modality Imaging[J]. Journal of Biomedical Nanotechnology,2008,4(4):474-481.
APA	Zhou, You-Fu.,Gulaka, Praveen.,Zhou, Jian.,Xiao, Ming.,Xu, Dongsheng.,...&Sun, Xiankai.(2008).Preparation and Evaluation of a Radioisotope-Incorporated Iron Oxide Core/Au Shell Nanoplatform for Dual Modality Imaging.Journal of Biomedical Nanotechnology,4(4),474-481.
MLA	Zhou, You-Fu,et al."Preparation and Evaluation of a Radioisotope-Incorporated Iron Oxide Core/Au Shell Nanoplatform for Dual Modality Imaging".Journal of Biomedical Nanotechnology 4.4(2008):474-481.