Selective protein immobilization via DNA conjugation under microfluidic laminar flow

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	Selective protein immobilization via DNA conjugation under microfluidic laminar flow
	Guo Su 1; Xue Mian-Qi 2; Qian Min-Xie 1; Cao Ting-Bing 2; Zhao Xin-Sheng 1
刊名	ACTA PHYSICO-CHIMICA SINICA
	2007-12-01
卷号	23 期号:12 页码:1827-1830
关键词	Microfluidic Device Proteinsensor Dna-directed Immobilization Total Internal Reflection Fluorescence
ISSN号	1000-6818
英文摘要	The feasibility of controlled protein immobilization via DNA conjugation by utilizing laminar flow in a microfluidic device was demonstrated. The glass surface in a microchannel was treated by oligonucleotides. The laminar flow brought different protein-DNA conjugates parallel into the microchannel. DNA recognition allows proteins to be delivered to the desired location. The total internal reflection fluorescence was also applied to monitor the process. Both the specificity and sensitivity were high, and the immobilization and removal of the proteins were repeatable. It was shown that with parallel detection of specific and non-specific recognitions, the accuracy of bioassay would be effectively enhanced. This strategy could improve the performing quality of biosensors in microfluidic devices.
语种	英语
出版者	PEKING UNIV PRESS
WOS记录号	WOS:000251812900001
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/62433]
专题	中国科学院化学研究所
通讯作者	Zhao Xin-Sheng
作者单位	1.Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, Beijing Natl Lab Mol Sci,State Key Lab Struct Che, Beijing 100871, Peoples R China 2.Renmin Unin China, Dept Chem, Beijing 100872, Peoples R China
推荐引用方式 GB/T 7714	Guo Su,Xue Mian-Qi,Qian Min-Xie,et al. Selective protein immobilization via DNA conjugation under microfluidic laminar flow[J]. ACTA PHYSICO-CHIMICA SINICA,2007,23(12):1827-1830.
APA	Guo Su,Xue Mian-Qi,Qian Min-Xie,Cao Ting-Bing,&Zhao Xin-Sheng.(2007).Selective protein immobilization via DNA conjugation under microfluidic laminar flow.ACTA PHYSICO-CHIMICA SINICA,23(12),1827-1830.
MLA	Guo Su,et al."Selective protein immobilization via DNA conjugation under microfluidic laminar flow".ACTA PHYSICO-CHIMICA SINICA 23.12(2007):1827-1830.