On-line solid-phase extraction with a monolithic weak cation-exchange column and simultaneous screening of alpha 1-adrenergin receptor antagonists in human plasma

doi:10.1002/jssc.200700342

CORC > 化学研究所 > 中国科学院化学研究所

	On-line solid-phase extraction with a monolithic weak cation-exchange column and simultaneous screening of alpha 1-adrenergin receptor antagonists in human plasma
	Wei, Xiaoyi ; Yin, Junfa ; Yang, Gengliang ; He, Chunxia ; Chen, Yi
刊名	JOURNAL OF SEPARATION SCIENCE
	2007-11-01
卷号	30 期号:17 页码:2851-2857
关键词	Alpha 1-adrenergic Receptor Antagonists Human Plasma Monolithic Weak Cation-exchange Column On-line Solid-phase Extraction
ISSN号	1615-9306
DOI	10.1002/jssc.200700342
英文摘要	An on-line SPE-HPLC method, using a monolithic poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-EDMA)) based weak cation-exchange (WCX) column, was developed for simultaneous determination of alpha 1-adrenergic receptor antagonists in human plasma. The monolithic WCX column was prepared by an in-situ polymerization protocol and modified stepwise with ethylenediamine and chloroacetic acid. On connecting this column to an injection valve, an on-line SPE protocol could be established for removal of matrices (mainly proteins and lipids) and preconcentration of four alpha 1-adrenergic receptor antagonists in human plasma. This method was validated and then used for determination of terazosin, alfuzosin, prazosin, and doxazosin in clinical plasma samples. For all analytes, each calibration curve was found to be linear over a range of 0.005 - 5 mu g/mL (R > 0.997), and the limit of detection for each analyte was 0.5 ng/mL. Recovery (> 80%) and precision (RSD < 15%) for inter- and intra-day assay were tested at three concentration levels of each analyte and showed acceptable results for quantitative assay. Real samples from hypertensive patients were monitored and results were in agreement with those of the conventional liquid-liquid extraction-HPLC method. Furthermore, due to its good permeability and biocompatibility, the monolithic WCX sorbent could be reused more than 300 times. The proposed method was especially appropriate for multi-analyte monitoring in plasma samples.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000251461500008
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/62323]
专题	中国科学院化学研究所
通讯作者	Yang, Gengliang
作者单位	1.Chinese Acad Sci, Inst Chem, Lab Analyt Chem Life Sci, Beijing Natl Lab Mol Sci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & Ecotoxicol, Beijing, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 4.Xiangtan Univ, Sch Chem, Xiangtan, Peoples R China
推荐引用方式 GB/T 7714	Wei, Xiaoyi,Yin, Junfa,Yang, Gengliang,et al. On-line solid-phase extraction with a monolithic weak cation-exchange column and simultaneous screening of alpha 1-adrenergin receptor antagonists in human plasma[J]. JOURNAL OF SEPARATION SCIENCE,2007,30(17):2851-2857.
APA	Wei, Xiaoyi,Yin, Junfa,Yang, Gengliang,He, Chunxia,&Chen, Yi.(2007).On-line solid-phase extraction with a monolithic weak cation-exchange column and simultaneous screening of alpha 1-adrenergin receptor antagonists in human plasma.JOURNAL OF SEPARATION SCIENCE,30(17),2851-2857.
MLA	Wei, Xiaoyi,et al."On-line solid-phase extraction with a monolithic weak cation-exchange column and simultaneous screening of alpha 1-adrenergin receptor antagonists in human plasma".JOURNAL OF SEPARATION SCIENCE 30.17(2007):2851-2857.