Synthesis of cross-linked enzyme aggregates (CLEAs) in CO2-expanded micellar solutions

doi:10.1016/j.colsurfb.2006.01.010

CORC > 化学研究所 > 中国科学院化学研究所

	Synthesis of cross-linked enzyme aggregates (CLEAs) in CO2-expanded micellar solutions
	Chen, J; Zhang, JL; Han, BX; Li, ZH; Li, JC; Feng, XY
刊名	COLLOIDS AND SURFACES B-BIOINTERFACES
	2006-03-01
卷号	48 期号:1 页码:72-76
关键词	Cleas Enzyme Reverse Micelles Co2
ISSN号	0927-7765
DOI	10.1016/j.colsurfb.2006.01.010
英文摘要	A new method to prepare the cross-linked enzyme aggregates (CLEAs) was developed. Through cross-linking the enzyme (Trypsin) aggregates, which was precipitated from the CO2-expanded reverse micellar solutions, dendritic CLEAs were obtained. The sizes of the CLEAs prepared by this new method were nanometer order of magnitudes and could be tuned by changing the water-to-surfactant ratio (w(0)) and the concentration of enzyme in the reverse micellar solution. The diameter of CLEAs increased with increasing w(0) value of reverse micelles and the concentration of Trypsin. The activity of CLEAs obtained by this method is improved in contrast to those obtained by the conventional method. This method has some advantages in applications and can be easily applied to the synthesis of other cross-linked enzyme aggregates. (c) 2006 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000236167800011
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/60241]
专题	中国科学院化学研究所
通讯作者	Han, BX
作者单位	Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Chen, J,Zhang, JL,Han, BX,et al. Synthesis of cross-linked enzyme aggregates (CLEAs) in CO2-expanded micellar solutions[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2006,48(1):72-76.
APA	Chen, J,Zhang, JL,Han, BX,Li, ZH,Li, JC,&Feng, XY.(2006).Synthesis of cross-linked enzyme aggregates (CLEAs) in CO2-expanded micellar solutions.COLLOIDS AND SURFACES B-BIOINTERFACES,48(1),72-76.
MLA	Chen, J,et al."Synthesis of cross-linked enzyme aggregates (CLEAs) in CO2-expanded micellar solutions".COLLOIDS AND SURFACES B-BIOINTERFACES 48.1(2006):72-76.