Hydrogen peroxide-induced chlorophyll a bleaching in the cytochrome b(6)f complex: a simple and effective assay for stability of the complex in detergent solutions

doi:10.1007/s11120-006-9118-0

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	Hydrogen peroxide-induced chlorophyll a bleaching in the cytochrome b(6)f complex: a simple and effective assay for stability of the complex in detergent solutions
	Chen, Xiao-Bo ; Zhao, Xiao-Hui ; Zhu, Yi ; Gong, Yan-Dao ; Li, Liang-Bi ; Zhang, Jian-Ping ; Kuang, Ting-Yun
刊名	PHOTOSYNTHESIS RESEARCH
	2006-12-01
卷号	90 期号:3 页码:205-214
关键词	Cytochrome b(6)f Detergent Chlorophyll Peroxidase Activity Hydrogen Peroxide
ISSN号	0166-8595
DOI	10.1007/s11120-006-9118-0
英文摘要	The instability of cytochrome b(6)f complex in detergent solutions is a well-known problem that has been studied extensively, but without finding a satisfactory solution. One of the important reasons can be short of the useful method to verify whether the complex suspended in different detergent is in an intact state or not. In this article, a simple and effective assay for stability of the complex was proposed based on the investigation on the different effects of the two detergents, n-octyl-beta-D-glucopyranoside (OG) and dodecyl-beta-D-maltoside (DDM), on the properties of the complex. DDM stabilizes the complex preparation more effectively whereas OG denatures the interactions of the heme groups and pigment molecules with the protein environment, leading to the bleaching of chlorophyll a induced by addition of hydrogen peroxide. The assay of the use of hydrogen peroxide to characterize the complex by studying the bleaching of chlorophyll induced by hydrogen peroxide and the peroxidase activity of the complex was discussed. This simple method will probably be useful to study the stability of the complex.
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000244725000002
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/59289]
专题	中国科学院化学研究所
通讯作者	Li, Liang-Bi
作者单位	1.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing 100093, Peoples R China 3.Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xiao-Bo,Zhao, Xiao-Hui,Zhu, Yi,et al. Hydrogen peroxide-induced chlorophyll a bleaching in the cytochrome b(6)f complex: a simple and effective assay for stability of the complex in detergent solutions[J]. PHOTOSYNTHESIS RESEARCH,2006,90(3):205-214.
APA	Chen, Xiao-Bo.,Zhao, Xiao-Hui.,Zhu, Yi.,Gong, Yan-Dao.,Li, Liang-Bi.,...&Kuang, Ting-Yun.(2006).Hydrogen peroxide-induced chlorophyll a bleaching in the cytochrome b(6)f complex: a simple and effective assay for stability of the complex in detergent solutions.PHOTOSYNTHESIS RESEARCH,90(3),205-214.
MLA	Chen, Xiao-Bo,et al."Hydrogen peroxide-induced chlorophyll a bleaching in the cytochrome b(6)f complex: a simple and effective assay for stability of the complex in detergent solutions".PHOTOSYNTHESIS RESEARCH 90.3(2006):205-214.