Characterization of a novel AP2/EREBP transcription factor TSH1 specifically binding to GCC elements

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	Characterization of a novel AP2/EREBP transcription factor TSH1 specifically binding to GCC elements
	Chen Feng ; Yu Jun-Ping ; Fang Xiao-Hong ; Zhang Gui-You
刊名	PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
	2006-07-01
卷号	33 期号:7 页码:627-634
关键词	Tsh1 Ap2/erebp Transcription Factor Afm
ISSN号	1000-3282
英文摘要	TSH1, a novel transcription factor in rice containing conserved AP2/EREBP domain, was screened from the EST database. The specific interaction of TSH1 and DNA elements was, detected by two kinds of traditional methods, electronic mobility assay and yeast one-hybrid assay, which suggested that THS1 specifically binds to GCC element in vitro and in vivo. Furthermore, It was confirmed by atom force microscopy (AFM) that TSH1 binds to GCC element (core sequence GCCGCC) not DRE element (core sequence GCCGAC), and the single molecular force between TSH1 and GCC element was quantitatively measured. The molecular force of TSH1 with GCC element is (83.9 +/- 2.2) pN. This interaction can be observably blocked by the dissociate TSH1 protein in the solution. These above results proved that TSH1 recognizes and binds to GCC with specifically interaction, which also demonstrate the high sensitivity of the AFM measurements for the single base substitution of the GCC core sequence greatly reduced the binding affinity. Comparing these methods for identification the binding of transcription factor with its DNA element, it was considered that AFM is expected to be a sensitive and reliable method that offers valuable information for the characterization of transcription factors.
语种	英语
出版者	SCIENCE CHINA PRESS
WOS记录号	WOS:000239288700004
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/58963]
专题	中国科学院化学研究所
通讯作者	Fang Xiao-Hong
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 2.Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Chen Feng,Yu Jun-Ping,Fang Xiao-Hong,et al. Characterization of a novel AP2/EREBP transcription factor TSH1 specifically binding to GCC elements[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2006,33(7):627-634.
APA	Chen Feng,Yu Jun-Ping,Fang Xiao-Hong,&Zhang Gui-You.(2006).Characterization of a novel AP2/EREBP transcription factor TSH1 specifically binding to GCC elements.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,33(7),627-634.
MLA	Chen Feng,et al."Characterization of a novel AP2/EREBP transcription factor TSH1 specifically binding to GCC elements".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 33.7(2006):627-634.