Signaling aptamer/protein binding by a molecular light switch complex

doi:10.1021/ac049565u

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	Signaling aptamer/protein binding by a molecular light switch complex
	Jiang, YX; Fang, XH; Bai, CL
刊名	ANALYTICAL CHEMISTRY
	2004-09-01
卷号	76 期号:17 页码:5230-5235
ISSN号	0003-2700
DOI	10.1021/ac049565u
英文摘要	A novel method of signaling aptamer/protein binding for aptamer-based protein detection has been developed using a molecular light switch complex, [Ru(phen)(2)-(dppz)](2+). The method takes advantage of the sensitive luminescence signal change of [Ru(phen)2(dppz)]2+ intercalating to the aptamer upon protein/aptamer binding. A 37-nt DNA aptamer against immunoglobulin E (IgE) was first tested as a model system. The luminescence of the [Ru(phen)(2)(dppz)](2+)/IgE aptamer decreased with the increase of IgE. By monitoring the luminescence change, we were able to detect the binding events between the aptamer and IgE for IgE quantitation in homogeneous solutions as well as in serum. The assay was highly selective and sensitive with a detection limit of 100 pM for IgE. This new method is very simple and without the need for the covalent coupling of fluorophores to aptamers. The generalizability of the method was demonstrated by the direct detection of two other proteins, oncoprotein platelet derived growth factor-BB (PDGF-BB) using its DNA aptamer and a-thrombin using its RNA aptamer. This new approach is expected to promote the exploitation of aptamer-based biosensors for protein assays in biochemical and biomedical studies.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000223733400062
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/55671]
专题	中国科学院化学研究所
通讯作者	Bai, CL
作者单位	Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
推荐引用方式 GB/T 7714	Jiang, YX,Fang, XH,Bai, CL. Signaling aptamer/protein binding by a molecular light switch complex[J]. ANALYTICAL CHEMISTRY,2004,76(17):5230-5235.
APA	Jiang, YX,Fang, XH,&Bai, CL.(2004).Signaling aptamer/protein binding by a molecular light switch complex.ANALYTICAL CHEMISTRY,76(17),5230-5235.
MLA	Jiang, YX,et al."Signaling aptamer/protein binding by a molecular light switch complex".ANALYTICAL CHEMISTRY 76.17(2004):5230-5235.