Synthesis and Self-Assembly of DNA-Aliphatic Polyether Dendron Hybrids

doi:10.6023/A13010087

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	Synthesis and Self-Assembly of DNA-Aliphatic Polyether Dendron Hybrids
	Zhao Zhiyong 1,3; Wu Fen 1; Yang Zhongqiang 2; Liu Dongsheng 2; Fan Qing-Hua 1
刊名	ACTA CHIMICA SINICA
	2013-04-15
卷号	71 期号:4 页码:549-554
关键词	Dna-dendron Amphiphile Self-assembly Spherical Micelle Nanofiber
ISSN号	0567-7351
DOI	10.6023/A13010087
英文摘要	A new kind of amphiphilic DNA-aliphatic polyether dendron hybrids consisting of a flexible hydrophobic polyether dendron and a single stranded DNA are synthesized, which are characterized by MALDI-TOF mass spectroscopy, HPLC and polyacrylamide gel electrophoresis (PAGE). In aqueous solution, as DNA length shortens from 24 mer to 18 mer, to 12 mer, to 6 mer, the hydrophilic DNA content in the DNA-aliphatic polyether dendron hybrid decreases, the morphology of the aggregates change from spherical micelles to nanofibers, and to irregular clusters. These different assemblies from DNA-aliphatic polyether dendron hybrids in aqueous solution are depended on the hydrophobic/hydrophilic ratio between the polyether dendron skeleton and DNA strand. However, when adding 1/10 (VIV) organic solvents such as dichloromethane (DCM), diethyl ether (EtOEt) or tetrahydrofuran (THF) into aqueous solution and after the assembling process that the sample solution is heated to 90 degrees C for 30 min and subsequently cooled to room temperature overnight, the third generation dendron conjugated 18 mer DNA hybrid could assemble into nanofibers. Meanwhile, in the THF/H2O (1: 10, V/Y) mixed solvents, with the same assembling process, as different dendron generations (the second or third generation) and different DNA lengths (6 mer, 12 mer, 18 mer or 24 mer) in the hybrids, all these hybrids could assemble into long nanofibers. The assembled structures have been characterized by transmission electron microscopy (TEM), atomic force microscope (AFM), dynamic light scattering (DLS) and fluorescent experiments. Subsequently, we verified the assembling mechanism that the spherical micelles and nanofibers contain a hydrophobic dendron core and a hydrophilic DNA shell by hydrophobic fluorescent molecule Nile Red encapsulation experiment and precise DNA hybridization to load gold nanoparticles at a size of 10 nm. The hybridization property of DNA at the shell associated with the encapsulation ability of dendron at the inner core indicated the potential application in gene or drug delivery and nanotechnology.
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000318258800011
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/53925]
专题	中国科学院化学研究所
通讯作者	Zhao Zhiyong
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci BNLMS, CAS Key Lab Mol Recognit & Funct, Inst Chem, Beijing 100190, Peoples R China 2.Tsinghua Univ, Key Lab Organ Optoelect & Mol Engn, Minist Educ, Dept Chem, Beijing 100084, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao Zhiyong,Wu Fen,Yang Zhongqiang,et al. Synthesis and Self-Assembly of DNA-Aliphatic Polyether Dendron Hybrids[J]. ACTA CHIMICA SINICA,2013,71(4):549-554.
APA	Zhao Zhiyong,Wu Fen,Yang Zhongqiang,Liu Dongsheng,&Fan Qing-Hua.(2013).Synthesis and Self-Assembly of DNA-Aliphatic Polyether Dendron Hybrids.ACTA CHIMICA SINICA,71(4),549-554.
MLA	Zhao Zhiyong,et al."Synthesis and Self-Assembly of DNA-Aliphatic Polyether Dendron Hybrids".ACTA CHIMICA SINICA 71.4(2013):549-554.