Ultrasensitive in Vivo Detection of Primary Gastric Tumor and Lymphatic Metastasis Using Upconversion Nanoparticles

doi:10.1021/nn507433p

CORC > 化学研究所 > 中国科学院化学研究所

	Ultrasensitive in Vivo Detection of Primary Gastric Tumor and Lymphatic Metastasis Using Upconversion Nanoparticles
	Qiao, Ruirui 1; Liu, Changhao 2; Liu, Muhan 3; Hu, Hao 2; Liu, Chunyan 1; Hou, Yi 1; Wu, Kaichun 2; Lin, Yenan 3; Liang, Jimin 3; Gao, Mingyuan 1
刊名	ACS NANO
	2015-02-01
卷号	9 期号:2 页码:2120-2129
关键词	Ultrasensitive Detection Gastric Cancer Lymphatic Metastasis Upconversion Nanoparticles Molecular Imaging
ISSN号	1936-0851
DOI	10.1021/nn507433p
英文摘要	Lymphatic metastasis is an important prognostic factor regarding long-term survival rate of gastric cancer (GC) patients. Pretreatment knowledge of lymph node status is extremely helpful for planning treatment and prognosis. However, to date, no imaging method has been demonstrated to be effective for detecting lymphatic metastasis in GC. Molecular imaging probes based on upconversion nanoparticles with unique optical and magnetic properties have provided great hope for early tumor detection. Herein we report highly sensitive detection of lymphatic spread using core@shell structured NaGdF4:Yb,Er@NaGdF4 upconversion nanoparticles coated with polyethylene glycol (PEG). A GC-specific probe was constructed through click reaction between the maleimide moiety of PEG ligand and the thiol group from partly reduced antigastric cancer antibody MGb(2). The primary tumor and adjacent lymphatic metastasis site were clearly differentiated by upconversion luminescence imaging after the GC-specific probe was delivered through tail vein injection into tumor-bearing mice. Moreover, lymphatic metastases smaller than 1 mm were successfully detected, owing to the ultralow background under 980 nm excitation. It has been demonstrated that both primary and lymphatic metastatic sites in an orthotopic mouse model of human gastric cancer can be optically detected by using GC-specific upconversion luminescence nanoprobes. The current studies may therefore provide a highly effective approach for GC diagnosis.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000349940500108
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/53521]
专题	中国科学院化学研究所
通讯作者	Wu, Kaichun
作者单位	1.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 2.Fourth Mil Med Univ, Xijing Hosp Digest Dis, State Key Lab Canc Biol, Xian 710032, Peoples R China 3.Xidian Univ, Sch Life Sci & Technol, Life Sci Res Ctr, Xian 710071, Peoples R China
推荐引用方式 GB/T 7714	Qiao, Ruirui,Liu, Changhao,Liu, Muhan,et al. Ultrasensitive in Vivo Detection of Primary Gastric Tumor and Lymphatic Metastasis Using Upconversion Nanoparticles[J]. ACS NANO,2015,9(2):2120-2129.
APA	Qiao, Ruirui.,Liu, Changhao.,Liu, Muhan.,Hu, Hao.,Liu, Chunyan.,...&Gao, Mingyuan.(2015).Ultrasensitive in Vivo Detection of Primary Gastric Tumor and Lymphatic Metastasis Using Upconversion Nanoparticles.ACS NANO,9(2),2120-2129.
MLA	Qiao, Ruirui,et al."Ultrasensitive in Vivo Detection of Primary Gastric Tumor and Lymphatic Metastasis Using Upconversion Nanoparticles".ACS NANO 9.2(2015):2120-2129.