Poly(2-Vinyl-4,4-dimethylazlactone)-Functionalized Magnetic Nanoparticles as Carriers for Enzyme Immobilization and Its Application

doi:10.1021/am5063025

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	Poly(2-Vinyl-4,4-dimethylazlactone)-Functionalized Magnetic Nanoparticles as Carriers for Enzyme Immobilization and Its Application
	Mu, Xiaoyu 1,2; Qiao, Juan 1; Qi, Li 1; Dong, Ping 3,4; Ma, Huimin 1
刊名	ACS APPLIED MATERIALS & INTERFACES
	2014-12-20
卷号	6 期号:23 页码:21346-21354
关键词	Magnetic Nanoparticles Enzyme Immobilization L-asparaginase Poly(2-vinyl-4 4-dimethylazlactone)
ISSN号	1944-8244
DOI	10.1021/am5063025
英文摘要	Fabrication of various efficient enzyme reactors has triggered increasing interests for its extensive applications in biological and clinical research. In this study, magnetic nanoparticles were functionalized by a biocompatible reactive polymer, poly(2-vinyl-4,4-dimethylazlactone), which was synthesized by reversible addition-fragmentation chain transfer polymerization. Then, the prepared polymer-modified magnetic nanoparticles were employed as favorable carriers for enzyme immobilization. l-Asparaginase was selected as the model enzyme to fabricate the enzyme reactor, and the prepared enzyme reactor exhibited high loading capacity of 318.0 mu g mg(-1) magnetic nanoparticle. Interestingly, it has been observed that the enzymolysis efficiency increased slightly with the lengthened polymer chain, resulting from the increased immobilization amount of enzyme. Meanwhile, the immobilized enzyme could retain more than 95.7% activity after 10 repeated uses and maintain more than 72.6% activity after 10 weeks storage. Moreover, an extracorporeal shunt system was simulated to estimate the potential application capability of the prepared l-asparaginase reactor in acute lymphoblastic leukemia treatment.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000346326600092
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/53253]
专题	中国科学院化学研究所
通讯作者	Qi, Li
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Chinese Acad Med Sci, Res Ctr Plast Surg Hosp, Beijing 100144, Peoples R China 4.Peking Union Med Coll, Beijing 100144, Peoples R China
推荐引用方式 GB/T 7714	Mu, Xiaoyu,Qiao, Juan,Qi, Li,et al. Poly(2-Vinyl-4,4-dimethylazlactone)-Functionalized Magnetic Nanoparticles as Carriers for Enzyme Immobilization and Its Application[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(23):21346-21354.
APA	Mu, Xiaoyu,Qiao, Juan,Qi, Li,Dong, Ping,&Ma, Huimin.(2014).Poly(2-Vinyl-4,4-dimethylazlactone)-Functionalized Magnetic Nanoparticles as Carriers for Enzyme Immobilization and Its Application.ACS APPLIED MATERIALS & INTERFACES,6(23),21346-21354.
MLA	Mu, Xiaoyu,et al."Poly(2-Vinyl-4,4-dimethylazlactone)-Functionalized Magnetic Nanoparticles as Carriers for Enzyme Immobilization and Its Application".ACS APPLIED MATERIALS & INTERFACES 6.23(2014):21346-21354.