Kinetics of blue-green light-induced state transition and fluorescence quenching in the wild-type cyanobacterium Synechocystis PCC 6803 and apcD (-) and apcF (-) mutants

doi:10.1007/s11434-014-0533-x

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	Kinetics of blue-green light-induced state transition and fluorescence quenching in the wild-type cyanobacterium Synechocystis PCC 6803 and apcD (-) and apcF (-) mutants
	Zhao, Wenfeng; Xie, Jie; Zhao, Jingquan
刊名	CHINESE SCIENCE BULLETIN
	2014-12-01
卷号	59 期号:34 页码:4712-4719
关键词	Cyanobacteria Mutants State Transitions Fluorescence Quenching 77 k Fluorescence Kinetics
ISSN号	1001-6538
DOI	10.1007/s11434-014-0533-x
英文摘要	State transition and blue-green light-induced fluorescence quenching are two short-term processes in cyanobacteria. The details of their kinetics and the relationship between these processes have not been elucidated. In this work, these two processes were studied in the wild-type cyanobacterium Synechocystis PCC 6803 cells as well as in apcD (-) and apcF (-) mutants by monitoring their time-dependent 77 K fluorescence responses to blue-green light (430-540 nm) at a series of intensities ranging from 20-800 A mu E m(-2) s(-1). The lowest light intensity to induce fluorescence quenching in wild-type cells was 160 A mu E m(-2) s(-1) under the selected experimental conditions, while state transition took place at the intensities lower than 160 A mu E m(-2) s(-1) at a conservative level, but at variable rates. The quenching level increased at intensities higher than 160 A mu E m(-2) s(-1), reaching the maximum level at intensities equal to or higher than 200 A mu E m(-2) s(-1). Fluorescence kinetics indicated that both the length of the induction period and time required to reach the maximum level were functions of light intensity. State transitions as well as fluorescence quenching took place in both wild-type and mutant cells, but might involve different mechanisms.
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000344614800009
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/53177]
专题	中国科学院化学研究所
通讯作者	Zhao, Jingquan
作者单位	Chinese Acad Sci, Beijing Natl Lab Mol Sci BNLMS, Inst Chem, CAS Key Lab Photochem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Wenfeng,Xie, Jie,Zhao, Jingquan. Kinetics of blue-green light-induced state transition and fluorescence quenching in the wild-type cyanobacterium Synechocystis PCC 6803 and apcD (-) and apcF (-) mutants[J]. CHINESE SCIENCE BULLETIN,2014,59(34):4712-4719.
APA	Zhao, Wenfeng,Xie, Jie,&Zhao, Jingquan.(2014).Kinetics of blue-green light-induced state transition and fluorescence quenching in the wild-type cyanobacterium Synechocystis PCC 6803 and apcD (-) and apcF (-) mutants.CHINESE SCIENCE BULLETIN,59(34),4712-4719.
MLA	Zhao, Wenfeng,et al."Kinetics of blue-green light-induced state transition and fluorescence quenching in the wild-type cyanobacterium Synechocystis PCC 6803 and apcD (-) and apcF (-) mutants".CHINESE SCIENCE BULLETIN 59.34(2014):4712-4719.