Asymmetric copolymer vesicles to serve as a hemoglobin vector for ischemia therapy

doi:10.1039/c4bm00123k

CORC > 化学研究所 > 中国科学院化学研究所

	Asymmetric copolymer vesicles to serve as a hemoglobin vector for ischemia therapy
	Li, Bin 1,2; Qi, Yanxin 1; He, ShaSha 1,2; Wang, Yupeng 1; Xie, Zhigang 1; Jing, Xiabin 1; Huang, Yubin 1
刊名	BIOMATERIALS SCIENCE
	2014
卷号	2 期号:9 页码:1254-1261
ISSN号	2047-4830
DOI	10.1039/c4bm00123k
英文摘要	Self-aggregated vesicles have been considered to be promising candidates for hemoglobin-based oxygen carriers. Here, amphiphilic hetero-triblock copolymers are designed and synthesized with the capacity to self-assemble into polymer vesicles (polymersomes). Conceivably, vesicles are formed with asymmetric membranes, which achieve enhanced encapsulation efficiency of hemoglobin (Hb) beyond the diblock counterpart. Furthermore, hemoglobin-loaded vesicles (HbV) are fabricated with high Hb content and submicron particle sizes. The gas-binding capability, oxygen affinity and methemoglobin (metHb) level of the HbV dispersions are all comparable to the natural erythrocytes. In vitro HbV stability studies further reveal that the encapsulation of Hb within vesicles can greatly avoid the existence of free Hb and shows no interference with cells, especially for blood components. To evaluate the efficacy on ischemia reperfusion, HbV suspended in a plasma expander is transfused as a resuscitation fluid into an acute anemia rat model. Results demonstrate that the combined infusion of the plasma expander with HbV effectively ameliorates the lethal shock symptom and reduces short-term mortality. Concurrently, rats transfused with HbV are void of the acute tubular necrosis caused by the filtration of dissociated Hb dimers from glomeruli. We envision that the oxygen carriers derived from polymer self-assembly technology will become an alternative strategy for future development of blood substitutes.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000341519500011
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/52675]
专题	中国科学院化学研究所
通讯作者	Li, Bin
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Li, Bin,Qi, Yanxin,He, ShaSha,et al. Asymmetric copolymer vesicles to serve as a hemoglobin vector for ischemia therapy[J]. BIOMATERIALS SCIENCE,2014,2(9):1254-1261.
APA	Li, Bin.,Qi, Yanxin.,He, ShaSha.,Wang, Yupeng.,Xie, Zhigang.,...&Huang, Yubin.(2014).Asymmetric copolymer vesicles to serve as a hemoglobin vector for ischemia therapy.BIOMATERIALS SCIENCE,2(9),1254-1261.
MLA	Li, Bin,et al."Asymmetric copolymer vesicles to serve as a hemoglobin vector for ischemia therapy".BIOMATERIALS SCIENCE 2.9(2014):1254-1261.