Continuous monitoring of bisulfide variation in microdialysis effluents by on-line droplet-based microfluidic fluorescent sensor

doi:10.1016/j.bios.2013.12.056

CORC > 化学研究所 > 中国科学院化学研究所

	Continuous monitoring of bisulfide variation in microdialysis effluents by on-line droplet-based microfluidic fluorescent sensor
	Zhu, Xiaocui 1; Xu, Lei 1; Wu, Tongbo 1; Xu, Anqin 1; Zhao, Meiping 1; Liu, Shaorong 2
刊名	BIOSENSORS & BIOELECTRONICS
	2014-05-15
卷号	55 页码:438-445
关键词	Hydrogen Sulfide Gold Nanoparticle Droplet-based Microfluidics Microdialysis Effluents On-line Detection
ISSN号	0956-5663
DOI	10.1016/j.bios.2013.12.056
英文摘要	We demonstrate a novel fluorescent sensor for real-time and continuous monitoring of the variation of bisulfide in microdialysis effluents by using a nanoparticle-glutathione-fluorescein isothiocyanate (AuNP-GSH-FITC) probe coupled with on-line droplet-based microfluidic chip. The AuNP-GSH-FITC fluorescent probe was firstly developed and used for bisulfide detection in bulk solution by quantitative real-time PCR, which achieved a linear working range from 0.1 mu M to 5.0 mu M and a limit of detection of similar to 50 nM. The response time was less than 2 min. With the aid of co-immobilized thiol-polyethylene glycol, the probe exhibited excellent stability and reproducibility in high salinity solutions, including artificial cerebrospinal fluids (aCSF). By adding 0.1% glyoxal to the probe solution, the assay allowed quantification of bisulfide in the presence of cysteine at the micro-molarity level. Using the AuNP-GSH-FITC probe, a droplet-based microfluidic fluorescent sensor was further constructed for online monitoring of bisulfide variation in the effluent of microdialysis. By using fluorescence microscope-charge-coupled device camera as the detector, the integrated microdialysis/microfluidic chip device achieved a detection limit of 2.0 mu M and a linear response from 5.0 p.M to 50 mu M for bisulfide in the tested sample. The method was successfully applied for the on-line measurement of bisulfide variation in aCSF and serum samples. It will be a very useful tool for tracking the variation of bisulfide or hydrogen sulfide in extracellular fluids. (C) 2013 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER ADVANCED TECHNOLOGY
WOS记录号	WOS:000332426100071
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/50913]
专题	中国科学院化学研究所
通讯作者	Zhao, Meiping
作者单位	1.Peking Univ, Beijing Natl Lab Mol Sci, MOE Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Univ Oklahoma, Dept Chem & Biochem, Norman, OK 73019 USA
推荐引用方式 GB/T 7714	Zhu, Xiaocui,Xu, Lei,Wu, Tongbo,et al. Continuous monitoring of bisulfide variation in microdialysis effluents by on-line droplet-based microfluidic fluorescent sensor[J]. BIOSENSORS & BIOELECTRONICS,2014,55:438-445.
APA	Zhu, Xiaocui,Xu, Lei,Wu, Tongbo,Xu, Anqin,Zhao, Meiping,&Liu, Shaorong.(2014).Continuous monitoring of bisulfide variation in microdialysis effluents by on-line droplet-based microfluidic fluorescent sensor.BIOSENSORS & BIOELECTRONICS,55,438-445.
MLA	Zhu, Xiaocui,et al."Continuous monitoring of bisulfide variation in microdialysis effluents by on-line droplet-based microfluidic fluorescent sensor".BIOSENSORS & BIOELECTRONICS 55(2014):438-445.