A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality

doi:10.1039/c4cc05554c

CORC > 化学研究所 > 中国科学院化学研究所

	A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality
	Zhou, Zhenpeng 1; Li, Tian 1; Huang, Hongduan 1; Chen, Yang 1; Liu, Feng 1; Huang, Chengzhi 2; Li, Na 1
刊名	CHEMICAL COMMUNICATIONS
	2014-11-11
卷号	50 期号:87 页码:13373-13376
ISSN号	1359-7345
DOI	10.1039/c4cc05554c
英文摘要	Silver-enhanced fluorescence was coupled with a bio-barcode assay to facilitate a dual amplification assay to demonstrate a non-enzymatic approach for simple and sensitive detection of DNA. In the assay design, magnetic nanoparticles seeded with silver nanoparticles were modified with the capture DNA, and silver nanoparticles were modified with the binding of ssDNA and the fluorescently labeled barcode dsDNA. Upon introduction of the target DNA, a sandwich structure was formed because of the hybridization reaction. By simple magnetic separation, silver-enhanced fluorescence of barcode DNAs could be readily measured without the need of a further step to liberate barcode DNAs from silver nanoparticles, endowing the method with simplicity and high sensitivity with a detection limit of 1 pM.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000343965700046
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/50073]
专题	中国科学院化学研究所
通讯作者	Huang, Chengzhi
作者单位	1.Peking Univ, Coll Chem & Mol Engn, Inst Analyt Chem,Minist Educ, BNLMS,Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China 2.Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence & Real Time Anal, Educ Minist, Chongqing 400715, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Zhenpeng,Li, Tian,Huang, Hongduan,et al. A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality[J]. CHEMICAL COMMUNICATIONS,2014,50(87):13373-13376.
APA	Zhou, Zhenpeng.,Li, Tian.,Huang, Hongduan.,Chen, Yang.,Liu, Feng.,...&Li, Na.(2014).A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality.CHEMICAL COMMUNICATIONS,50(87),13373-13376.
MLA	Zhou, Zhenpeng,et al."A dual amplification strategy for DNA detection combining bio-barcode assay and metal-enhanced fluorescence modality".CHEMICAL COMMUNICATIONS 50.87(2014):13373-13376.