PEGylation of bovine serum albumin using click chemistry for the application as drug carriers

doi:10.1002/btpr.1526

CORC > 化学研究所 > 中国科学院化学研究所

	PEGylation of bovine serum albumin using click chemistry for the application as drug carriers
	Li, Xiao-Yuan 1; Li, Tai-Hang 1,2; Guo, Jin-Shan 1; Wei, Ying 1,2; Jing, Xia-Bin 1; Chen, Xue-Si 1; Huang, Yu-Bin 1
刊名	BIOTECHNOLOGY PROGRESS
	2012-05-01
卷号	28 期号:3 页码:856-861
关键词	Bovine Serum Albumin Mpeg Click Chemistry Rifampicin 5-fluorouracil Drug Carriers
ISSN号	8756-7938
DOI	10.1002/btpr.1526
英文摘要	Monomethyl poly(ethylene glycol) (mPEG)-modified bovine serum albumin (BSA) conjugates (BSA-mPEG) were obtained by the mild Cu(I)-mediated cycloaddition reaction of azided BSA (BSA-N3) and alkyne-terminated mPEG. The structure and characteristics of BSA-mPEG conjugates were thoroughly investigated. There were about two PEG chains conjugated onto each BSA molecule as determined by matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) analysis. The intrinsic nonspecific binding ability of BSA was used for adsorption and sustained release of both rifampicn and 5-fluorouracil (5-FU). The helical structures of BSA were preserved to a large extent after modification and drug adsorption on BSA was confirmed via circular dichroism spectroscopy. Drugs adsorbed onto the conjugated formulation to a lesser extent than on BSA due to mPEG modification. The in vitro release of both rifampicin and 5-FU, however, indicated that BSA-mPEG can function as a drug carrier. Overall, the click reaction provided a convenient tool for the pegylation of BSA. The biological activity of the BSA-mPEG conjugates, including the drug transportation capacity and biocompatibility, were largely retained. (C) 2012 American Institute of Chemical Engineers Biotechnol. Prog.,, 2012
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000304989800026
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/49565]
专题	中国科学院化学研究所
通讯作者	Huang, Yu-Bin
作者单位	1.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Changchun Inst Appl Chem, Chinese Acad Sci, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
推荐引用方式 GB/T 7714	Li, Xiao-Yuan,Li, Tai-Hang,Guo, Jin-Shan,et al. PEGylation of bovine serum albumin using click chemistry for the application as drug carriers[J]. BIOTECHNOLOGY PROGRESS,2012,28(3):856-861.
APA	Li, Xiao-Yuan.,Li, Tai-Hang.,Guo, Jin-Shan.,Wei, Ying.,Jing, Xia-Bin.,...&Huang, Yu-Bin.(2012).PEGylation of bovine serum albumin using click chemistry for the application as drug carriers.BIOTECHNOLOGY PROGRESS,28(3),856-861.
MLA	Li, Xiao-Yuan,et al."PEGylation of bovine serum albumin using click chemistry for the application as drug carriers".BIOTECHNOLOGY PROGRESS 28.3(2012):856-861.