Regulation of Glucose Oxidase Activity through Interaction with Fullerene Derivatives

doi:10.1002/cjoc.201180479

CORC > 化学研究所 > 中国科学院化学研究所

	Regulation of Glucose Oxidase Activity through Interaction with Fullerene Derivatives
	Gao Yunyan 1,2; Wang Zhongli 1,2; Ou Zhize 1,2; Li Yi 3; Wang Xuesong 3; Yang Guoqiang 4
刊名	CHINESE JOURNAL OF CHEMISTRY
	2012-02-01
卷号	30 期号:2 页码:418-426
关键词	Fullerene C60 Glucose Oxidase Partial Mixed-type Inhibition Mechanism Redox Mediator Electron Transfer
ISSN号	1001-604X
DOI	10.1002/cjoc.201180479
英文摘要	The 2-(hydroxymethyl)pyridine modified C60 (PY-C60) and methoxydiglycol modified C60 (MDG-C60) are synthesized using Bingel-Hirsch reaction and characterized by nuclear magnetic resonance (NMR) and mass spectra. PY-C60 and MDG-C60 can bind to glucose oxidase (GOx) and quench the fluorescence of tryptophan (Trp) residue in GOx through static mechanism. The conformation of GOx is disturbed after formation of complex with these fullerene derivatives. Kinetic analysis indicates that PY-C60 and MDG-C60 may affect the catalytic activity of GOx with a partial mixed-type inhibition mechanism. In the plasma glucose concentration range (3.65.2 mmol center dot L-1), PY-C60 may significantly accelerate the catalytic velocity of GOx, however, MDG-C60 exerts almost no obvious change to the initial velocity of GOx, suggesting that elaborate design of molecular structure of fullerene derivative is very important for regulating the biological activity of fullerene-enzyme complex.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000300674000039
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/49209]
专题	中国科学院化学研究所
通讯作者	Gao Yunyan
作者单位	1.NW Polytech Univ, Minist Educ, Key Lab Space Appl Phys & Chem, Xian 710072, Shaanxi, Peoples R China 2.NW Polytech Univ, Sch Sci, Dept Appl Chem, Xian 710072, Shaanxi, Peoples R China 3.Chinese Acad Sci, Tech Inst Phys & Chem, Beijing 100190, Peoples R China 4.Chinese Acad Sci, Inst Chem, CAS Key Lab Photochem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Gao Yunyan,Wang Zhongli,Ou Zhize,et al. Regulation of Glucose Oxidase Activity through Interaction with Fullerene Derivatives[J]. CHINESE JOURNAL OF CHEMISTRY,2012,30(2):418-426.
APA	Gao Yunyan,Wang Zhongli,Ou Zhize,Li Yi,Wang Xuesong,&Yang Guoqiang.(2012).Regulation of Glucose Oxidase Activity through Interaction with Fullerene Derivatives.CHINESE JOURNAL OF CHEMISTRY,30(2),418-426.
MLA	Gao Yunyan,et al."Regulation of Glucose Oxidase Activity through Interaction with Fullerene Derivatives".CHINESE JOURNAL OF CHEMISTRY 30.2(2012):418-426.