Receptor-Mediated Delivery of Magnetic Nanoparticles across the Blood-Brain Barrier

doi:10.1021/nn300240p

CORC > 化学研究所 > 中国科学院化学研究所

	Receptor-Mediated Delivery of Magnetic Nanoparticles across the Blood-Brain Barrier
	Qiao, Ruirui 2; Jia, Qiaojuan 2; Huewel, Sabine 3; Xia, Rui 1; Liu, Ting 1; Gao, Fabao 1; Galla, Hans-Joachim 3; Gao, Mingyuan 2
刊名	ACS NANO
	2012-04-01
卷号	6 期号:4 页码:3304-3310
关键词	Magnetic Nanoparticle Blood-brain Barrier Lactoferrin Receptor-mediated Mri
ISSN号	1936-0851
DOI	10.1021/nn300240p
英文摘要	A brain delivery probe was prepared by covalently conjugating lactoferrin (Lf) to a poly(ethylene glycol) (PEG)-coated Fe3O4 nanoparticle in order to facilitate the transport of the nanoparticles across the blood-brain barrier (BBB) by receptor-mediated transcytosis via the Lf receptor present on cerebral endothelial cells. The efficacy of the Fe3O4-Lf conjugate to cross the BBB was evaluated in vitro using a cell culture model for the blood-brain barrier as well as in vivo in SD rats. For an in vitro experiment, a well-established porcine BBB model was used based on the primary culture of cerebral capillary endothelial cells grown on filter supports, thus allowing one to follow the transfer of nanoparticles from the apical (blood) to the basolateral (brain) side. For in vivo experiments, SD rats were used as animal model to detect the passage of the nanoparticles through the BBB by MRI techniques. The results of both in vitro and in vivo experiments revealed that the Fe3O4-Lf probe exhibited an enhanced ability to cross the BBB in comparison to the PEG-coated Fe3O4 nanoparticles and further suggested that the Lf-receptor-mediated transcytosis was an effective measure for delivering the nanoparticles across the BBB.
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000303099300052
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/47885]
专题	中国科学院化学研究所
通讯作者	Gao, Fabao
作者单位	1.Sichuan Univ, W China Hosp, Dept Radiol, Mol Imaging Lab, Chengdu 610041, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 3.Univ Munster, Inst Biochem, D-48149 Munster, Germany
推荐引用方式 GB/T 7714	Qiao, Ruirui,Jia, Qiaojuan,Huewel, Sabine,et al. Receptor-Mediated Delivery of Magnetic Nanoparticles across the Blood-Brain Barrier[J]. ACS NANO,2012,6(4):3304-3310.
APA	Qiao, Ruirui.,Jia, Qiaojuan.,Huewel, Sabine.,Xia, Rui.,Liu, Ting.,...&Gao, Mingyuan.(2012).Receptor-Mediated Delivery of Magnetic Nanoparticles across the Blood-Brain Barrier.ACS NANO,6(4),3304-3310.
MLA	Qiao, Ruirui,et al."Receptor-Mediated Delivery of Magnetic Nanoparticles across the Blood-Brain Barrier".ACS NANO 6.4(2012):3304-3310.