A spectroscopic off-on probe for simple and sensitive detection of carboxylesterase activity and its application to cell imaging

doi:10.1039/c2an15952j

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	A spectroscopic off-on probe for simple and sensitive detection of carboxylesterase activity and its application to cell imaging
	Zhang, Yangyang; Chen, Wei; Feng, Duan; Shi, Wen; Li, Xiaohua; Ma, Huimin
刊名	ANALYST
	2012
卷号	137 期号:3 页码:716-721
ISSN号	0003-2654
DOI	10.1039/c2an15952j
英文摘要	A new resorufin-based spectroscopic probe, 7-(p-acetoxyphenylmethyloxy)-3H-phenoxazin-3-one (1), has been developed for detecting carboxylesterase activity. The probe is designed by introducing p-acetoxyphenylmethyloxy as a bifunctional moiety to resorufin. The p-acetoxyphenylmethyloxy moiety not only quenches the spectroscopic signal of resorufin, but also serves as a recognition unit for carboxylesterase. As a result, the prepared latent spectroscopic probe 1 shows very low background signal, which is rather desired for achieving sensitive detection. The specific cleavage of the carboxylic ester bond by carboxylesterase induces the hydrolysis of the probe, resulting in the release of resorufin and thereby the recovery of both color and fluorescence signal. This behaviour leads to the development of a simple and sensitive fluorescent method for assaying carboxylesterase activity, with a detection limit of 8.6 x 10(-5) U mL(-1), which is much more sensitive than the existing fluorescence approaches. Moreover, experimental results showed that the probe 1 is cell membrane permeable, and its applicability has been demonstrated for monitoring carboxylesterase activity in HeLa cells.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000298969000028
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/47639]
专题	中国科学院化学研究所
通讯作者	Shi, Wen
作者单位	Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yangyang,Chen, Wei,Feng, Duan,et al. A spectroscopic off-on probe for simple and sensitive detection of carboxylesterase activity and its application to cell imaging[J]. ANALYST,2012,137(3):716-721.
APA	Zhang, Yangyang,Chen, Wei,Feng, Duan,Shi, Wen,Li, Xiaohua,&Ma, Huimin.(2012).A spectroscopic off-on probe for simple and sensitive detection of carboxylesterase activity and its application to cell imaging.ANALYST,137(3),716-721.
MLA	Zhang, Yangyang,et al."A spectroscopic off-on probe for simple and sensitive detection of carboxylesterase activity and its application to cell imaging".ANALYST 137.3(2012):716-721.