On-line electrophoretic sample clean-up for sensitive and reproducible mu CE immunoassay

doi:10.1039/c2lc21111d

CORC > 化学研究所 > 中国科学院化学研究所

	On-line electrophoretic sample clean-up for sensitive and reproducible mu CE immunoassay
	Pan, Qiong 1; Hong, Soongweon 2; Zhu, Xiaocui 1; Zhao, Meiping 1; Lee, Luke P.2
刊名	LAB ON A CHIP
	2012
卷号	12 期号:5 页码:932-938
ISSN号	1473-0197
DOI	10.1039/c2lc21111d
英文摘要	We report a novel on-line electrophoretic sample clean-up approach for highly sensitive and reproducible microchip electrophoretic (mu CE) immunoassay of low-abundance proteins in human serum. The method takes advantage of the differential effect of field-amplified sample stacking on molecules with different electrophoretic mobility. Large interfering proteins are removed from the loading channel by simple voltage control, resulting in selective concentration and injection of smaller target analytes to the separation channel. As a proof of concept, an antibody-free injection mode was developed for direct mu CE immunoassay of human insulin-like growth factor-I (IGF-I) in serum samples without any additional purification steps. Clear and sharp peaks were obtained for IGF-I with low background and excellent reproducibility. Besides, the assay sensitivity was further increased by addition of ethanol to the sample buffer at a concentration of 50% right before performing the mu CE detection. The lower limit of detection of IGF-I achieved 0.68 ng mL(-1), with an overall signal enhancement factor of 2750. The established on-line electrophoretic sample clean-up approach may find wide applications in the development of other microchip-based high-throughput analytical platforms for clinical and biological use.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000300047800012
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/47279]
专题	中国科学院化学研究所
通讯作者	Zhao, Meiping
作者单位	1.Peking Univ, Beijing Natl Lab Mol Sci, MOE Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Univ Calif Berkeley, Berkeley Sensor & Actuator Ctr, Dept Bioengn, Berkeley, CA 94720 USA
推荐引用方式 GB/T 7714	Pan, Qiong,Hong, Soongweon,Zhu, Xiaocui,et al. On-line electrophoretic sample clean-up for sensitive and reproducible mu CE immunoassay[J]. LAB ON A CHIP,2012,12(5):932-938.
APA	Pan, Qiong,Hong, Soongweon,Zhu, Xiaocui,Zhao, Meiping,&Lee, Luke P..(2012).On-line electrophoretic sample clean-up for sensitive and reproducible mu CE immunoassay.LAB ON A CHIP,12(5),932-938.
MLA	Pan, Qiong,et al."On-line electrophoretic sample clean-up for sensitive and reproducible mu CE immunoassay".LAB ON A CHIP 12.5(2012):932-938.