A New Fluorescence Turn-on Cyanide Sensor with Fluorescein-labeled Oligo DNA-Cu(II) Ensemble

doi:10.6023/A12080554

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	A New Fluorescence Turn-on Cyanide Sensor with Fluorescein-labeled Oligo DNA-Cu(II) Ensemble
	Huang Xianhong; Zhang Guanxin; Zhang Deqing
刊名	ACTA CHIMICA SINICA
	2012-10-28
卷号	70 期号:20 页码:2133-2136
关键词	Cyanide Oligo Dna Copper Ion Fluorescence Sensor
ISSN号	0567-7351
DOI	10.6023/A12080554
英文摘要	In this paper, a new fluorescence turn-on sensor for cyanide in aqueous solution was established with fluorescein-labeled oligo DNA. The design rationale is explained as follows: (1) Fluorescein-labeled oligoDNA (FAM-DNA) shows strong fluorescence in aqueous solution. But, it is expected that the fluorescence of FAM-DNA will be quenched because of the formation of stable complex of FAM-DNA with Cu2+; (2) Demetallation from the complex of FAM-DNA with Cu2+ because of the reaction of CN- and Cu2+ and formation Cu(CN)(2)(-); as a result the fluorescence of FAM-DNA emerges again. Therefore, it is anticipated that the fluorescence of the ensemble of FAM-DNA and Cu2+ increases by increasing the concentration of CN-. Thus, the ensemble of FAM-DNA and Cu2+ can be utilized for the detection of CN-. The results revealed that the fluorescence of FAM-DNA were quenched efficiently in the presence of Cu2+ (1.35 mu mol/L) due to the intramolecular photoinduced electron transfer within the ensemble of FAM-DNA and Cu2+. Other metal ions, such as K+, Ca2+, Ba2+, Mg2+, Fe3+, Ni2+, Pb2+, Zn2+ and Ag+, induced negligible fluorescence quenching for LAM-DNA under the same conditions. However, fluorescence of the system increased gradually by increasing the concentration of CN- in the ensemble solution, and the fluorescence intensity varies almost linearly vs. the concentration of CN-. The detection limit of CN- with the ensemble of FAM-DNA and Cu2+ was estimated to be 0.02 mu mol/L, which is much lower than the maximum level (1.9 mu mol/L) of CN- in drinking water permitted by the World Health Organization (WHO). Moreover, the response of the ensemble of FAM-DNA and Cu2+ to solutions containing CN- (40 mu mol/L) in the presence of relevant ionic species, including F-, Cl-, Br-, l(-), NO3-, HSO4-, CO32-, CHCOO-, H2PO4-, SCN-, and N-3(-) (at a concentration level over 10 times higher to that of CN), was investigated. The results indicated no significant interference effect was found. Furthermore, the experiments conducted in various water samples spiked with cyanide confirmed the potential practical application of this probe in real samples.
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000311533600005
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/47249]
专题	中国科学院化学研究所
通讯作者	Huang Xianhong
作者单位	Chinese Acad Sci, Organ Solids Lab, Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Huang Xianhong,Zhang Guanxin,Zhang Deqing. A New Fluorescence Turn-on Cyanide Sensor with Fluorescein-labeled Oligo DNA-Cu(II) Ensemble[J]. ACTA CHIMICA SINICA,2012,70(20):2133-2136.
APA	Huang Xianhong,Zhang Guanxin,&Zhang Deqing.(2012).A New Fluorescence Turn-on Cyanide Sensor with Fluorescein-labeled Oligo DNA-Cu(II) Ensemble.ACTA CHIMICA SINICA,70(20),2133-2136.
MLA	Huang Xianhong,et al."A New Fluorescence Turn-on Cyanide Sensor with Fluorescein-labeled Oligo DNA-Cu(II) Ensemble".ACTA CHIMICA SINICA 70.20(2012):2133-2136.