Fluorescence correlation spectroscopy of repulsive systems: Theory, simulation, and experiment

doi:10.1063/1.4807860

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	Fluorescence correlation spectroscopy of repulsive systems: Theory, simulation, and experiment
	Feng, Ligang 1,3; Yang, Jingfa 1; Zhao, Jiang 1; Wang, Dapeng 2; Koynov, Kaloian 2; Butt, Hans-Juergen 2
刊名	JOURNAL OF CHEMICAL PHYSICS
	2013-06-07
卷号	138 期号:21
ISSN号	0021-9606
DOI	10.1063/1.4807860
英文摘要	The theoretical basis of fluorescence correlation spectroscopy (FCS) for repulsive systems, such as charged colloids or macromolecules, has been further expanded and developed. It is established that the collective correlation function can no longer be fitted using the theoretical model of non-interacting systems. Also, it is discovered that the collective correlation function can be divided into two parts: a self-part and a distinct-part, named as the self-correlation and cross-correlation function, respectively. The former indicates the self-diffusion of objects, while the latter describes mutual interactions. Dual-color fluorescence cross-correlation spectroscopy provides the direct measurements of the two parts. The particle concentration and mean squared displacement of single particles can be deduced from the self-correlation function, while the correlation volume between particles can be approximated from the cross-correlation function. In the case of charged colloids, the Debye length of the solution and particle surface charge number can be fitted from the cross-correlation function. These theoretical results are successfully proven using Brownian dynamics simulations and preliminary FCS experiments for model charged colloidal systems. (C) 2013 AIP Publishing LLC.
语种	英语
出版者	AMER INST PHYSICS
WOS记录号	WOS:000320138900047
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/46769]
专题	中国科学院化学研究所
通讯作者	Zhao, Jiang
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100190, Peoples R China 2.Max Planck Inst Polymer Res, D-55128 Mainz, Germany 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Feng, Ligang,Yang, Jingfa,Zhao, Jiang,et al. Fluorescence correlation spectroscopy of repulsive systems: Theory, simulation, and experiment[J]. JOURNAL OF CHEMICAL PHYSICS,2013,138(21).
APA	Feng, Ligang,Yang, Jingfa,Zhao, Jiang,Wang, Dapeng,Koynov, Kaloian,&Butt, Hans-Juergen.(2013).Fluorescence correlation spectroscopy of repulsive systems: Theory, simulation, and experiment.JOURNAL OF CHEMICAL PHYSICS,138(21).
MLA	Feng, Ligang,et al."Fluorescence correlation spectroscopy of repulsive systems: Theory, simulation, and experiment".JOURNAL OF CHEMICAL PHYSICS 138.21(2013).