Biocompatible TiO2 nanoparticle-based cell immunoassay for circulating tumor cells capture and identification from cancer patients

doi:10.1007/s10544-013-9781-9

CORC > 化学研究所 > 中国科学院化学研究所

	Biocompatible TiO2 nanoparticle-based cell immunoassay for circulating tumor cells capture and identification from cancer patients
	He, Rongxiang 1; Zhao, Libo 3,4; Liu, Yumin 1; Zhang, Nangang 1; Cheng, Boran 2; He, Zhaobo 1; Cai, Bo 1; Li, Sizhe 1; Liu, Wei 1; Guo, Shishang 1
刊名	BIOMEDICAL MICRODEVICES
	2013-08-01
卷号	15 期号:4 页码:617-626
关键词	Microfluidics Tio2 Nanoparticles Biocompatible Circulating Tumor Cells
ISSN号	1387-2176
DOI	10.1007/s10544-013-9781-9
英文摘要	We demonstrate the isolation of circulating tumor cells (CTCs) with a biocompatible nano-film composed of TiO2 nanoparticles. Due to the enhanced topographic interaction between nano-film and cancer cell surface, cancer cells (HCT116) spiked into PBS and healthy blood can be recovered from the suspension, whose efficiencies were respectively 80 % and 50 %. Benifit from the biocompatibility of this nano-film, in-situ culture of the captured cancer cells is also available, which provides an alternative selection when the capture cell number was inadequate or the sample cannot be analyzed immediately. For the proof-of-concept study, we use this nano-film to separate the circulating tumor cells from the colorectal and gastric cancer patient peripheral blood samples and the captured CTCs are identified by a three-colored immunocytochemistry method. We investigated the cancer cells capture strength at the nano-bio interface through exposing the cells to fluid shear stress in microfluidic device, which can be utilized to increase the purity of CTCs. The result indicated that 50 % of the captured cells can be detached from the substrate when the fluid shear stress was 180 dyn cm(-2). By integration of this CTCs capture nano-film with other single cell analysis device, we expected to further explore their applications in genome sequencing based on the captured CTCs.
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000323909200005
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/46673]
专题	中国科学院化学研究所
通讯作者	Zhao, Xing-Zhong
作者单位	1.Wuhan Univ, Key Lab Artificial Micro & Nanostruct, Minist Educ, Sch Phys & Technol, Wuhan 430072, Peoples R China 2.Wuhan Univ, Zhongnan Hosp, Hubei Key Lab Tumor Biol Behav, Hubei Canc Clin Study Ctr, Wuhan 430071, Peoples R China 3.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Mol Nanostruct & Nanotechnol, Inst Chem, Beijing 100190, Peoples R China 4.Univ Calif Los Angeles, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA 5.Ecole Normale Super, Dept Chem, F-75231 Paris, France
推荐引用方式 GB/T 7714	He, Rongxiang,Zhao, Libo,Liu, Yumin,et al. Biocompatible TiO2 nanoparticle-based cell immunoassay for circulating tumor cells capture and identification from cancer patients[J]. BIOMEDICAL MICRODEVICES,2013,15(4):617-626.
APA	He, Rongxiang.,Zhao, Libo.,Liu, Yumin.,Zhang, Nangang.,Cheng, Boran.,...&Zhao, Xing-Zhong.(2013).Biocompatible TiO2 nanoparticle-based cell immunoassay for circulating tumor cells capture and identification from cancer patients.BIOMEDICAL MICRODEVICES,15(4),617-626.
MLA	He, Rongxiang,et al."Biocompatible TiO2 nanoparticle-based cell immunoassay for circulating tumor cells capture and identification from cancer patients".BIOMEDICAL MICRODEVICES 15.4(2013):617-626.