An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase

doi:10.1002/chem.201701384

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	An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase
	Wang, Ping 1; Xia, Yang-Liu 2; Zou, Li-Wei 1; Qian, Xing-Kai 2; Dou, Tong-Yi 2; Jin, Qiang 1; Li, Shi-Yang 2; Yu, Yang 2; Wang, Dan-Dan 1; Luo, Qun 3
刊名	CHEMISTRY-A EUROPEAN JOURNAL
	2017-08-10
卷号	23 期号:45 页码:10800-10807
关键词	Bio-imaging Biosensing Catechol-o-methyltransferase Coumarins Fluorescence
ISSN号	0947-6539
DOI	10.1002/chem.201701384
英文摘要	A practical two-photon fluorescent probe was developed for highly sensitive and selective sensing of the activities of catechol-O-methyltransferase (COMT) in complex biological samples. To this end, a series of 3-substituted 7,8-dihydroxycoumarins were designed and synthesized. Among them, 3-BTD displayed the best combination of selectivity, sensitivity, reactivity, and fluorescence response following COMT-catalyzed 8-O-methylation. The newly developed two-photon fluorescent probe 3-BTD can be used for determining the activities of COMT in complex biological samples and bio-imaging of endogenous COMT in living cells and tissue slices with good cell permeability, low cytotoxicity, and high imaging resolution. All these findings suggest that 3-BTD holds great promise for developing therapeutic molecules that target COMT, as well as for exploring COMT-associated biological processes and its biological functions in living systems. Furthermore, the strategy also sheds new light on the development of fluorescent probes for other conjugative enzymes.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000407397300015
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/44969]
专题	中国科学院化学研究所
通讯作者	Ge, Guang-Bo; Yang, Ling
作者单位	1.Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Natl Ctr Mass Spectrometry Beijing, CAS Key Lab Analyt Chem Living Biosyst,Inst Chem, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Wang, Ping,Xia, Yang-Liu,Zou, Li-Wei,et al. An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase[J]. CHEMISTRY-A EUROPEAN JOURNAL,2017,23(45):10800-10807.
APA	Wang, Ping.,Xia, Yang-Liu.,Zou, Li-Wei.,Qian, Xing-Kai.,Dou, Tong-Yi.,...&Yang, Ling.(2017).An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase.CHEMISTRY-A EUROPEAN JOURNAL,23(45),10800-10807.
MLA	Wang, Ping,et al."An Optimized Two-Photon Fluorescent Probe for Biological Sensing and Imaging of Catechol-O-Methyltransferase".CHEMISTRY-A EUROPEAN JOURNAL 23.45(2017):10800-10807.