Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering

doi:10.1007/s11426-014-5159-y

CORC > 化学研究所 > 中国科学院化学研究所

	Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering
	Niu Lin 1,2; Yan JingJing 1,2; Yang XuYan 1,2; Burger, Christian 3; Rong LiXia 3; Hsiao, Benjamin 3; Liang DeHai 1,2
刊名	SCIENCE CHINA-CHEMISTRY
	2014-12-01
卷号	57 期号:12 页码:1738-1745
关键词	Phase Behavior Dna Surfactant Synchrotron Small-angle X-ray Scattering Kinetics
ISSN号	1674-7291
DOI	10.1007/s11426-014-5159-y
英文摘要	The phase behaviors of the complex formed by didodecyldimethylammonium bromide (DDAB) and cetyltrimethylammonium bromide (CTAB) interacting with three different types of DNAs, salmon testes DNA (similar to 2000 bp), 21-bp double-stranded oligonucleotides (oligo-dsDNA), and 21-nt single-stranded oligonucleotides (oligo-ssDNA) were studied by synchrotron small-angle X-ray scattering. It was found that the DNA length and flexibility, together with the positive/negative charge ratio, determined the final structure. At higher charge ratios, the DNA length exhibited negligible effect. Both oligo-dsDNA and salmon DNA formed inverted hexagonal packing of cylinders with CTAB, as well as bilayered lamella with DDAB. However, at lower charge ratios, oligo-dsDNA formed a distorted hexagonal phase with CTAB and a new structure with DDAB, which was different from the behaviors of salmon DNA. The flexible oligo-ssDNA formed rich structures that were subject to environmental disturbance. Kinetic study also indicated that the structures of the complex formed by oligo-ssDNA took much longer to mature than the structures formed by oligo-dsDNA. We attributed this result to the conformational adjustment of oligo-ssDNA in the complex.
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000346047500017
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/43535]
专题	中国科学院化学研究所
通讯作者	Liang DeHai
作者单位	1.Peking Univ, Minist Educ, Key Lab Polymer Chem & Phys, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Peking Univ, Coll Chem & Mol Engn, Dept Polymer Sci & Engn, Beijing 100871, Peoples R China 3.SUNY Stony Brook, Dept Chem, Stony Brook, NY 11794 USA
推荐引用方式 GB/T 7714	Niu Lin,Yan JingJing,Yang XuYan,et al. Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering[J]. SCIENCE CHINA-CHEMISTRY,2014,57(12):1738-1745.
APA	Niu Lin.,Yan JingJing.,Yang XuYan.,Burger, Christian.,Rong LiXia.,...&Liang DeHai.(2014).Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering.SCIENCE CHINA-CHEMISTRY,57(12),1738-1745.
MLA	Niu Lin,et al."Complexation of DNA with cationic surfactants as studied by small-angle X-ray scattering".SCIENCE CHINA-CHEMISTRY 57.12(2014):1738-1745.