Sensitive quantification of isoprenoid cytokinins in plants by selective immunoaffinity purification and high performance liquid chromatography-quadrupole-time of flight mass spectrometry

doi:10.1016/j.ymeth.2011.08.006

CORC > 化学研究所 > 中国科学院化学研究所

	Sensitive quantification of isoprenoid cytokinins in plants by selective immunoaffinity purification and high performance liquid chromatography-quadrupole-time of flight mass spectrometry
	Liang, Yuan; Zhu, Xiaocui; Zhao, Meiping; Liu, Huwei
刊名	METHODS
	2012-02-01
卷号	56 期号:2 页码:174-179
关键词	Isoprenoid Cytokinins Immunoaffinity Extraction High Performance Liquid Chromatography-quadrupole-time Of Flight Mass Spectrometry Plant Tissue
ISSN号	1046-2023
DOI	10.1016/j.ymeth.2011.08.006
英文摘要	In this work, we developed a novel class-specific immunoaffinity column for the natural isoprenoid cytokinins (CTKs) by using trans-zeatin riboside as the hapten to generate a complete antigen. By combination with a mixed-mode solid phase extraction step for pre-cleanup and a high performance liquid chromatography-quadrupole-time of flight mass spectrometry for the quantification, an efficient analytical protocol was established which allowed simultaneous quantification of eight endogenous isoprenoid CTKs in Arabidopsis thaliana leaves with a wide linear range from 25 to 500 pg/g fresh weight and a detection limit of 12.5 pg/g fresh weight. The method will be very useful for comprehensive research on the networks of signaling interactions of the active phytohormones and their regulation of the plant functions. (C) 2011 Elsevier Inc. All rights reserved.
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000302105400009
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/43437]
专题	中国科学院化学研究所
通讯作者	Zhao, Meiping
作者单位	Peking Univ, Beijing Natl Lab Mol Sci, MOE Key Lab Bioorgan Chem & Mol Engn, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Liang, Yuan,Zhu, Xiaocui,Zhao, Meiping,et al. Sensitive quantification of isoprenoid cytokinins in plants by selective immunoaffinity purification and high performance liquid chromatography-quadrupole-time of flight mass spectrometry[J]. METHODS,2012,56(2):174-179.
APA	Liang, Yuan,Zhu, Xiaocui,Zhao, Meiping,&Liu, Huwei.(2012).Sensitive quantification of isoprenoid cytokinins in plants by selective immunoaffinity purification and high performance liquid chromatography-quadrupole-time of flight mass spectrometry.METHODS,56(2),174-179.
MLA	Liang, Yuan,et al."Sensitive quantification of isoprenoid cytokinins in plants by selective immunoaffinity purification and high performance liquid chromatography-quadrupole-time of flight mass spectrometry".METHODS 56.2(2012):174-179.