Facile Fabrication of a Graphene-based Electrochemical Biosensor for Glucose Detection

doi:10.1002/cjoc.201100452

CORC > 化学研究所 > 中国科学院化学研究所

	Facile Fabrication of a Graphene-based Electrochemical Biosensor for Glucose Detection
	Zhang Yanqin 1; Fan Youjun 1; Wang Shanshan 1; Tan Yiliang 1; Shen Xingcan 1; Shi Zujin 2
刊名	CHINESE JOURNAL OF CHEMISTRY
	2012-05-01
卷号	30 期号:5 页码:1163-1167
关键词	Graphene Glucose Oxidase Direct Electrochemistry Biosensor Electroanalysis
ISSN号	1001-604X
DOI	10.1002/cjoc.201100452
英文摘要	A simple and effective glucose biosensor based on immobilization of glucose oxidase (GOD) in graphene (GR)/Nafion film was constructed. The results indicated that the immobilized GOD can maintain its native structure and bioactivity, and the GR/Nafion film provides a favorable microenvironment for GOD immobilization and promotes the direct electron transfer between the electrode substrate and the redox center of GOD. The electrode reaction of the immobilized GOD shows a reversible and surface-controlled process with the large electron transfer rate constant (ks) of 3.42 +/- 0.08 s-1. Based on the oxygen consumption during the oxidation process of glucose catalyzed by the immobilized GOD, the as-prepared GOD/GR/Nafion/GCE electrode exhibits a linear range from 0.5 to 14 mmol.L-1 with a detection limit of 0.03 mmol.L-1. Moreover, it displays a good reproducibility and long-term stability.
语种	英语
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000304045600025
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/43423]
专题	中国科学院化学研究所
通讯作者	Fan Youjun
作者单位	1.Guangxi Normal Univ, Key Lab Chem & Mol Engn Med Resources, Minist Educ China, Coll Chem & Chem Engn, Guilin 541004, Guangxi, Peoples R China 2.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Rare Earth Mat Chem & Applicat, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Zhang Yanqin,Fan Youjun,Wang Shanshan,et al. Facile Fabrication of a Graphene-based Electrochemical Biosensor for Glucose Detection[J]. CHINESE JOURNAL OF CHEMISTRY,2012,30(5):1163-1167.
APA	Zhang Yanqin,Fan Youjun,Wang Shanshan,Tan Yiliang,Shen Xingcan,&Shi Zujin.(2012).Facile Fabrication of a Graphene-based Electrochemical Biosensor for Glucose Detection.CHINESE JOURNAL OF CHEMISTRY,30(5),1163-1167.
MLA	Zhang Yanqin,et al."Facile Fabrication of a Graphene-based Electrochemical Biosensor for Glucose Detection".CHINESE JOURNAL OF CHEMISTRY 30.5(2012):1163-1167.