Metal-organic frameworks as affinity agents to enhance the microdialysis sampling efficiency of fatty acids

doi:10.1039/c8an00238j

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	Metal-organic frameworks as affinity agents to enhance the microdialysis sampling efficiency of fatty acids
	Yang, Hui 1,2; Li, Tuo 1,2; Liu, Lu 1,2; Li, Na 1,2; Guan, Ming 1,2; Zhang, Yangyang 1; Wang, Zhenpeng 1; Zhao, Zhenwen 1,2
刊名	ANALYST
	2018-05-07
卷号	143 期号:9 页码:2157-2164
ISSN号	0003-2654
DOI	10.1039/c8an00238j
英文摘要	Microdialysis (MD) has been extensively used for in vivo sampling of hydrophilic analytes such as neurotransmitters and drug metabolites. In contrast, there have been few reports on sampling of lipophilic analytes by MD. Lipophilic analytes are easily adsorbed on the surfaces of the dialysis membrane and the inner wall of tubing, which leads to a very low relative recovery (RR). In this work, a strategy to develop an enhanced MD sampling of fatty acids (FAs) by using metal-organic frameworks (MOFs) as affinity agents in the perfusion fluid was investigated. Two MOFs, MIL-101 and ZIF-8, were synthesized and tested for the first time. A 2 times higher RR, about 70% RR, was obtained. The FT-IR experiment showed that the unsaturated metal sites in MOFs could coordinate with FAs, therefore the FAs were encapsulated into MOFs, avoiding FAs to be absorbed on the surfaces of the dialysis membrane and the inner wall of tubing. Moreover, incorporation of FAs into MOFs led to a decrease of free concentration of FAs inside the MD membrane and an increase of concentration gradient, allowing more FAs to diffuse across the membrane. Consequentially, an enhanced RR was obtained. The approach was successfully used to monitor the time profile of targeted FAs in cell culture media after lipopolysaccharide (LPS)-induced inflammation.
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000431109400025
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/43191]
专题	中国科学院化学研究所
通讯作者	Zhao, Zhenwen
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci,Inst Chem,Beijing Mass S, CAS Res Educ Ctr Excellence Mol Sci, Key Lab Analyt Chem Living Biosyst, Beijing, Peoples R China 2.Univ Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Yang, Hui,Li, Tuo,Liu, Lu,et al. Metal-organic frameworks as affinity agents to enhance the microdialysis sampling efficiency of fatty acids[J]. ANALYST,2018,143(9):2157-2164.
APA	Yang, Hui.,Li, Tuo.,Liu, Lu.,Li, Na.,Guan, Ming.,...&Zhao, Zhenwen.(2018).Metal-organic frameworks as affinity agents to enhance the microdialysis sampling efficiency of fatty acids.ANALYST,143(9),2157-2164.
MLA	Yang, Hui,et al."Metal-organic frameworks as affinity agents to enhance the microdialysis sampling efficiency of fatty acids".ANALYST 143.9(2018):2157-2164.