Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study

doi:10.1016/j.chroma.2018.03.031

CORC > 化学研究所 > 中国科学院化学研究所

	Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study
	Zhao, Liping 1,4; Qiao, Juan 1,2; Zhang, Ke 2,3; Li, Dan 5; Zhang, Hongyi 4; Qi, Li 1,2
刊名	JOURNAL OF CHROMATOGRAPHY A
	2018-05-04
卷号	1548 页码:104-110
关键词	Chiral Ligand Exchange Capillary Electrochromatography Enantioseparation Block Copolymer Coating Enzyme Kinetics Study
ISSN号	0021-9673
DOI	10.1016/j.chroma.2018.03.031
英文摘要	A chiral ligand exchange capillary electrochromatography (CLE-CEC) protocol was designed and implemented for D,L-amino acids enantioseparation with poly(maleic anhydride-styrene-methacryloylL-arginine methyl ester) as the coating. The block copolymer was synthesized through the reversible addition fragmentation chain transfer reaction. In the constructed CLE-CEC system, poly (methacryloylL-arginine methyl ester) moiety of the block copolymer played the role as the immobilized chiral ligand and Zn (II) was used as the central ion. Key factors, including pH of buffer solution, ratio of Zn (II) to ligands, the mass ratio of monomers in the block copolymer, which affect the enantioresolution were investigated. Comparing with the bare capillary, the CLE-CEC enantioresolution was enhanced greatly with the coating one. 5 Pairs of D,L-amino acids enantiomers obtained baseline separation with 5 pairs partly separated. The mechanism of enhancement enantioresolution of the developed CLE-CEC system was explored briefly. Further, good linearities were achieved in the range of 25.0 mu M-5.0 mM for quantitative analysis of D-glutamine (r(2) = 0.997) and L-glutamine (r(2) = 0.991). Moreover, the proposed CLE-CEC assay was successfully applied in the kinetics study of glutaminase by using L-glutamine as the substrate. (C) 2018 Elsevier B.V. All rights reserved.
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000430779500013
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/43131]
专题	中国科学院化学研究所
通讯作者	Zhang, Hongyi; Qi, Li
作者单位	1.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, 2 Zhongguancun Beiyijie, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Chem, State Key Lab Polymer Phys & Chem, 2 Zhongguancun Beiyijie, Beijing 100190, Peoples R China 4.Hebei Univ, Key Lab Analyt Sci & Technol Hebei Prov, Coll Chem & Environm Sci, 180 Wusi East Rd, Baoding 071002, Peoples R China 5.Jinan Univ, Guangzhou 510632, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Liping,Qiao, Juan,Zhang, Ke,et al. Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1548:104-110.
APA	Zhao, Liping,Qiao, Juan,Zhang, Ke,Li, Dan,Zhang, Hongyi,&Qi, Li.(2018).Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study.JOURNAL OF CHROMATOGRAPHY A,1548,104-110.
MLA	Zhao, Liping,et al."Construction of chiral ligand exchange capillary electrochromatography for D,L-amino acids enantioseparation and its application in glutaminase kinetics study".JOURNAL OF CHROMATOGRAPHY A 1548(2018):104-110.